Gene expression refers to the process in which cells convert genetic information stored in DNA sequences into biologically active protein molecules in the process of life to perform various functions. Genes that are actively expressed generally account for about 10-15% of the genes contained in the genome, and researchers even overexpress or suppress the target fragments. Analyzing the expression of these genes has become an important part of life science research.
Creative BioMart Biomarker provides you with different technical means, such as fluorescence quantitative PCR and transcriptome sequencing (RNA-seq), etc., which can study the RNA in various samples under various conditions and the changes of DNA related to expression regulation at different levels.
Protein level expression detection can be divided into Western Blot, ELISA and HPLC-based quantitative detection analysis and protein function analysis. Protein function analysis can be divided into the following parts:
Detection of mRNA expression levels can be divided into:
Gene expression analysis of animal samples, plant samples, bacteria and fungi samples; expression profile chip, transcriptome sequencing results verification; RNAi interference target gene test results
Verification of microRNA chip and microRNA sequencing experiment results
Analysis of the expression of non-coding RNA of various lengths in various tissues of humans and mice
Analysis of copy number changes of specific genes in the genome, or absolute quantification of the total number of microorganisms; qualitative detection of viruses and pathogens, qualitative identification of biological species.
At the same time, we provide accurate quantitative gene expression services. Only a specific lysate is required to lyse the sample, and specific particles can specifically recognize the target mRNA. The signal intensity is proportional to the amount of mRNA, and the gene can be accurately quantified by detecting the fluorescence intensity.
The experimental method used to detect the expression level and size of eukaryotic RNA to estimate its abundance can obtain data simultaneously through a large number of experimental samples.
The Protocol of Northern Blot:
Add a fluorescent group to the PCR system, use the change of the fluorescent signal to detect the change of each amplification product in the PCR amplification reaction in real time, and quantitatively analyze the starting template through the relationship between the Ct value and the standard curve or internal reference gene.
The Protocol of qRT-PCR:
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