Semi-quantitative RT-PCR Service

In the life sciences, advances in molecular biology have paved the way for innovative research and diagnostics. Semi-quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a technique that has received much attention in biomarker research. Creative BioMart Biomarker has been utilizing semi-quantitative RT-PCR for many years to unlock the potential of biomarker research.

What is Semi-quantitative RT-PCR?

Semi-quantitative RT-PCR is a powerful molecular biology tool that allows researchers to assess and compare the relative expression levels of specific genes in biological samples. The technique combines reverse transcription and PCR to convert RNA molecules into complementary DNA (cDNA) and amplify specific target genes. Unlike quantitative real-time PCR (qRT-PCR), semi-quantitative RT-PCR does not provide absolute quantification, but provides valuable insight into changes in gene expression between samples.

Semi-quantitative RT-PCR Service Offered by Creative BioMart Biomarker

• Sample Preparation: The success of semi-quantitative RT-PCR relies heavily on the isolation of high-quality RNA from biological samples. At Creative BioMart Biomarker, we employ robust RNA extraction methods to ensure RNA integrity and purity.
• cDNA Synthesis: The isolated RNA is reverse-transcribed, and reverse transcriptase converts the RNA to cDNA. We utilize advanced RT enzymes and protocols for efficient and reliable cDNA synthesis.
• Primer Design and Assay Setup: Our expert team designs specific primers that flank regions of interest in the target gene. These primers are rigorously optimized to ensure specificity and sensitivity. After the detection setup is complete, we run a trial run to verify the performance of the detection.
• PCR Amplification: PCR amplification of cDNA along with optimized primers using our advanced thermal cycler. Careful control of cycle numbers and conditions ensures that PCR remains in exponential phase for accurate quantitation.
• Gel Electrophoresis and Analysis: After PCR, amplicons are separated and visualized using gel electrophoresis. The obtained bands represent the presence and relative abundance of the target gene. Our gel recording systems capture high-resolution images for analysis.
• Data Interpretation and Reporting: At Creative BioMart Biomarker, data analysis is a critical step in biomarker research. Our skilled experts analyze data, compare gene expression levels between samples and identify potential candidate biomarkers. The findings are then compiled into a detailed report to the client.

Application of Semi-quantitative RT-PCR in Biomarker Research

• Identify potential biomarkers of various cancers and analyze gene expression patterns in cancerous and healthy tissues.
• Accurately detect and quantify the presence of viruses, bacteria, or other infectious agents in clinical samples by targeting pathogen-specific genes.
• Find biomarkers associated with neurodegenerative diseases.
• For studying gene expression changes associated with cardiovascular disease.

Why Choose Creative BioMart Biomarker for Semi-quantitative RT-PCR Service?

• State-of-the-art laboratory equipment ensures a high level of precision and accuracy for semi-quantitative RT-PCR.
• The expertise of our team of scientists and researchers in experimental design, primer optimization and data analysis guarantees reliable results.
• Design custom semi-quantitative RT-PCR to ensure optimal gene targeting and accurate data interpretation.
• Rigorous quality assurance measures are implemented at every step of the semiquantitative RT-PCR workflow to ensure reproducible and reliable results.
• Comprehensive data analysis and interpretation services provide clients with meaningful insights.

Workflow of Semi-quantitative RT-PCR Service

Creative BioMart Biomarker is an ideal partner for biomarker research. Through our customized solutions and comprehensive data analysis, we can accelerate customers' scientific discovery and progress in the field of precision medicine. If you are interested in our services, please feel free to contact us.