In Situ Hybridization, ISH

In Situ Hybridization (ISH) is a detection technique related to gene expression and localization. This technology uses the principle of base pairing in the process of RNA and DNA from denaturation to renaturation. The purpose is to make the biomarker RNA or DNA probe and the target RNA and DNA contained in the cell form RNA-RNA, RNA-DNA, DNA-DNA complex, and then through the amplification and collection of probe signals to analyze the monitoring related to nucleic acids.

The Protocol of ISH:

1. Probe design and preparation

Creative BioMart Biomarker provides DNA probes, cDNA probes, CRNA probes, and oligonucleotide probes with a variety of sensitivity and specific probe synthesis and preparation, as well as DIG, Biotin, fluorescein and other non-isotopic labels for choice.

2. Sample preparation and prehybridization

Creative BioMart Biomarker can provide paraffin-embedded tissue sections and frozen section services, section fixation and pretreatment services, and we provide a variety of anti-probe marker antibodies.

3. In situ hybridization

In addition to basic ISH hybridization, we can provide a variety of experimental services such as Fluorescence in situ hybridization (FISH) service based on ISH, Multicolor in situ hybridization, in situ PCR and genomic in situ hybridization technology, to provide genetic consultation and medical identification for you, Genetic disease identification and DNA specific function research and other services.

4. Post-hybridization treatment

5. Color development and observation

The ISH service provided by Creative BioMart Biomarker has the following advantages:

• Reagents and probes are economical, safe and stable, and can be used within two years after one labeling
• The experimental period is short, the results can be obtained quickly, the specificity is good, the positioning is accurate, and the sensitivity is high
• Multi-color FISH can simultaneously detect multiple sequences by displaying different colors in the same core
• An experimental protocol has been established for multiple ISH applications to enhance sensitivity and signal. It can display the number or structure of metaphase chromosomes on slides, or the structure of interphase chromosome DNA in suspension
• According to customer requirements, personalized experimental programs can be customized to control ISH experiments to be completed within 1-2 weeks

Our company has relatively mature molecular related experience and technology platform, which can reduce the complex and time-consuming process of ISH in your laboratory, at the same time can reduce the experimental cost and help your scientific research work. At the same time, you can customize the experimental plan according to your needs.