In situ PCR has garnered immense recognition and application in the realm of studying gene expression and localization within tissues. Its formidable potency and versatility have elevated it to a significant stature in the analysis of biomarkers and their intricate participation in sundry biological processes. Creative BioMart Biomarker's in situ PCR services are geared towards the detection of biomarkers, thus delivering valuable insights into the diagnosis, treatment, and prognosis of diseases.
In Situ PCR and Biomarkers
In situ PCR is designed to amplify DNA or RNA within cells, tissues, or organs while they are still in their native location. Common types of in situ PCR include direct in situ PCR, indirect in situ PCR, reverse transcriptase in situ PCR (RT in situ PCR). Thanks to in situ PCR, experts can now glean valuable insights into the localization of a particular gene, mRNA, or protein of interest. Its distinct advantage over conventional PCR lies in its capacity to detect the target sequence in its original location. As a result, researchers can pinpoint the exact location of a particular gene, mRNA, or protein within the tissue, thereby facilitating the study of biomarkers.
Figure 1. Diagram of the protocol of in situ RT-PCR combined with immunogold labeling for electron microscopy. (Cubas-Nuñez, L.; et al. 2017)
In Situ PCR Services
Our state-of-the-art in situ PCR services cater to the diverse needs of researchers and provide them with top-notch results that will propel their research to new heights of discovery.
Our comprehensive in situ PCR services comprise a wide range of specialized offerings, including the detection of specific genes, mRNA, or proteins in tissues. We provide customized protocols that are tailor-made to cater to the unique requirements of specific tissues or cell types, and also offer optimization and troubleshooting services to ensure that our clients achieve the best possible results.
The Flow of in Situ PCR
- Preparation of the tissue - The tissue or cell sample is first fixed, embedded, and sectioned onto a slide. The sections are then treated with protease to increase accessibility of the target sequence.
- Probe design - The probe must be specific to the target sequence, and the primers used for amplification must be carefully designed to ensure that they do not cross-react with other sequences in the tissue.
- Amplification - The PCR reagents are added to the tissue sections, and the amplification is carried out using a thermal cycler.
- Detection - The amplicons are detected using labeled probes, which allow for visualization of the amplified product.
Our Advantages
- Rich experience in in situ PCR and biomarker analysis, committed to providing high-quality, reliable results to meet the specific needs of customers.
- Possession of the latest technology to ensure services are accurate, efficient and cost-effective.
- Applicable to a wide range of sample types, customized solutions to meet the unique needs of customers.
Workflow of in Situ PCR Services
In situ PCR services provided by Creative BioMart Biomarker are designed to provide accurate and reliable results for a wide range of research applications. Contact us today to learn more about how our in situ PCR services can help you achieve your research goals.
Reference:
- Cubas-Nuñez, L.; et al. In situ RT-PCR Optimized for Electron Microscopy Allows Description of Subcellular Morphology of Target mRNA-Expressing Cells in the Brain. Front. Cell. Neurosci. 2017, 11:141.
