In the field of molecular biology, understanding protein interactions is critical to elucidating complex cellular processes and signaling pathways. Among the various techniques available, yeast two-hybrid (Y2H) stands out as a powerful tool for studying protein-protein interactions in living cells. Creative BioMart Biomarker has many years of industry experience and provides customers with Y2H services with unique advantages.
What is Y2H?
Y2H is a pioneering method that has revolutionized the study of protein interactions. It relies on the modular nature of transcriptional activators in yeast to detect interactions between two proteins of interest.
At Creative BioMart Biomarker, we design bait vectors and prey vectors, which contain a DNA-binding domain (DBD) and an activation domain (AD), respectively, fused to the protein of interest. When these vectors are introduced into yeast cells by transformation, the interaction between the bait protein and the prey protein brings DBD and AD into close proximity, thereby activating the reporter gene. This activation can serve as a readout for successful protein-protein interactions, allowing researchers to study and elucidate complex biological processes.
Figure 1. Schematic representation of the yeast two-hybrid system. (Heller, G.; et al. 2017)
Y2H Service Offered by Creative BioMart Biomarker
Creative BioMart Biomarker provides Y2H services tailored for biomarker research. Our team of experienced scientists and researchers is well versed in the intricacies of Y2H experiments, ensuring accurate and reliable results. With an extensive library of pre-validated baits and prey vectors, we expedite the experimental process and provide our customers with efficient and cost-effective solutions.
- Consulting and Experimental Design
After receiving the project, our experts conduct a detailed consultation with the client to understand the research objectives and requirements. Based on this information, we design a comprehensive experimental strategy that meets our client's goals.
Once the experimental design is complete, our team constructs the bait and prey vector. These vectors are optimized for the Y2H system.
- Yeast Cell Transformation
After vector construction, we performed yeast cell transformation to introduce bait and prey vectors into yeast cells.
Screening assays were performed on transformed yeast cells to detect protein interactions. Positive interactions were further validated by rigorously controlled experiments to eliminate false positives and ensure the accuracy of results.
- Data Analysis and Reporting
Analyze the obtained data and perform statistical analysis. Comprehensive reports detailing experiments, results, and interpretations are generated and delivered to clients.
Application of Y2H in Biomarker Research
- Identify novel protein interactions associated with specific diseases
- Uncover interactions between drug candidates and their potential cellular targets
- Validation of potential biomarker-associated interactions
- Mapping protein interactions in disease-relevant cellular pathways
Why Choose Creative BioMart Biomarker for Y2H Service?
- Custom Experimental Design: Design custom Y2H experiments targeting specific research objectives. Whether it is a disease-related or therapeutic biomarker study, our tailor-made approach ensures high efficiency and a high success rate.
- Strict Quality Control: We employ strict quality control measures at every step of the Y2H process, from vector construction to data analysis. This ensures accuracy and reproducibility of results, providing our clients with reliable insights.
- Extensive Data Analysis: By employing advanced algorithms and tools, we transform raw Y2H data into meaningful information, facilitating a deeper understanding of protein interactions and their impact in biomarker research.
Workflow of Y2H Service
If you are interested in our services, please feel free to contact us.
Reference:
- Heller, G., et al. Methods of probing the interactions between small molecules and disordered proteins. Cell Mol Life Sci. 2017, 74(17): 3225-3243.
