Real-time Quantitative PCR Service
In the rapidly evolving field of biomarker research, real-time quantitative PCR (qPCR) has emerged as a powerful tool, enabling researchers to gain valuable insights into gene expression levels and identify potential biomarkers for various diseases and conditions. As a leading company at the forefront of innovative scientific solutions, Creative BioMart Biomarker provides efficient and reliable qPCR services to facilitate biomarker research.
What is Real-time Quantitative PCR?
Real-time quantitative PCR, commonly referred to as qPCR or RT-qPCR, is a sophisticated molecular biology technique for the precise quantification of DNA or RNA in real time during amplification. Unlike traditional PCR, which detects amplification products only after the reaction is complete, qPCR continuously monitors the amplification reaction through the use of fluorescent probes. This ability to measure DNA or RNA levels in real time provides accurate quantitative data, making qPCR an indispensable tool in biomarker research.
qPCR Service Offered by Creative BioMart Biomarker
At Creative BioMart Biomarker, we take great pride in offering qPCR services tailored for biomarker research. Our expertise and dedication to delivering accurate, timely results sets us apart in the industry.
Performing qPCR requires a well-structured workflow to ensure accurate and reliable results.
Whether analyzing patient tissue, blood, or cell cultures, proper sample preparation is critical to obtaining reliable biomarker data.
We work with clients to design primers and fluorescent probes for target biomarkers of interest. These oligonucleotides are optimized for optimal qPCR results.
For RNA targets, reverse transcription is performed using reverse transcriptase to convert RNA to complementary DNA (cDNA).
Our skilled team prepares qPCR reaction mixes, including cDNA templates, primers, fluorescent probes, and DNA polymerases. This reaction mixture is then loaded onto the qPCR instrument.
- Thermal Cycling and Data Collection
The qPCR instrument cycles through a series of temperature changes, including denaturation, annealing, and extension. During each cycle, the fluorescent signal emitted by the probe is measured and recorded in real time.
After the qPCR run is complete, an amplification plot is generated and Ct values are calculated. Our experts will interpret the results.
Application of qPCR in Biomarker Research
- Identify and validate differentially expressed genes and noncoding RNAs associated with various cancer types.
- Identifying and quantifying pathogenic DNA or RNA in patient samples facilitates timely diagnosis and monitoring of infectious diseases.
- Analyze gene expression patterns associated with heart health and disease.
- Study gene expression changes in neural tissue to identify potential biomarkers for diseases such as Alzheimer's and Parkinson's diseases.
Why Choose Creative BioMart Biomarker for qPCR Service?
- Significant investments in new qPCR platforms and equipment ensure high levels of sensitivity, precision and data quality.
- A team of experienced experts ensures that every qPCR experiment is performed with high proficiency and meticulous attention to detail.
- Custom Assay Design. We work closely with our clients to design primer and probe sequences to guarantee reliable and accurate results.
- Implement strict quality control measures to ensure data accuracy and reproducibility.
Workflow of qPCR Service
Creative BioMart Biomarker's qPCR service provides valuable insights into gene expression. If you are interested in our services, please feel free to contact us.
