Two-dimensional Gel Electrophoresis

In the ever-growing field of biological research and diagnostics, proteomics has become a powerful tool for understanding the complex world of proteins and their roles in various diseases. Two-dimensional gel electrophoresis (2-DE) is the gold standard for protein separation and analysis. At Creative BioMart Biomarker, we have been utilizing 2-DE for biomarker research, providing our clients with cutting-edge services and expertise.

What is 2-DE?

2-DE is a technique that separates and analyzes proteins based on their isoelectric point (pI) and molecular weight. By combining two orthogonal separation methods, isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), 2-DE allows a comprehensive examination of the protein composition of a sample.

In the first dimension of 2-DE, IEF is used to separate proteins according to their pI. In the second dimension, proteins from the first dimension gel are subjected to SDS-PAGE to separate them according to molecular weight.

2-DE has proven to be a valuable technique for the discovery and analysis of molecular biomarkers. By comparing protein profiles between different samples, researchers can identify differentially expressed proteins that can serve as potential biomarkers.

2-DE Services Offered by Creative BioMart Biomarker

At Creative BioMart Biomarker, we harness the power of 2-DE in biomarker research to provide our clients with valuable insights into disease mechanisms and potential therapeutic targets. Our expert team utilizes state-of-the-art equipment and advanced analytical techniques to analyze protein expression profiles and identify significant changes in protein abundance. We provide customers with one-stop services from sample preparation to image analysis and protein identification, so as to realize the seamless connection of upstream and downstream research.

The Flow of 2-DE

Our Advantages

• Samples are collected and processed using optimized protocols to preserve protein integrity.
• Perform 2-DE experiments with high precision and efficiency.
• State-of-the-art equipment, including high-resolution gel imaging systems and advanced image analysis software.
• Strict quality control measures are employed to ensure reproducibility and accuracy.

At Creative BioMart Biomarker, we leverage the power of 2-DE to provide our clients with comprehensive proteomics services, facilitating the identification and characterization of potential biomarkers. With our advanced equipment, expertise and comprehensive approach to mass spectrometry, we enable customers to unlock proteomic insights. Please feel free to contact us to discuss further how you can accelerate your biomarker project by partnering with us.