Liquid Chromatography Service
Liquid chromatography (LC) is a powerful analytical technique that has revolutionized biomarker research, enabling the precise identification and quantification of molecular biomarkers in complex biological samples. With years of experience in the industry, Creative BioMart Biomarker has been using liquid chromatography for biomarker analysis, providing high-end services and delivering accurate and reliable results.
What is Liquid Chromatography?
Liquid chromatography (LC) is a separation technique that relies on the differential interaction of compounds with a stationary phase and a mobile phase. It involves injecting a sample into a chromatographic system where the components of the sample are separated based on their affinity for the stationary phase.
The application of LC in Biomarker Studies
Biomarkers are molecular entities, such as proteins, peptides, metabolites, nucleic acids, and other small molecules, that exhibit altered levels or modifications in response to physiological changes or pathological conditions. Liquid chromatography has become an essential tool in biomarker research because of its ability to detect and quantify these biomarkers with high sensitivity and specificity.
LC Services Offered by Creative BioMart Biomarker
Creative BioMart Biomarker is a company with extensive expertise in biomarker analysis, offering cutting-edge LC services tailored to the specific needs of biomarker research. Our team of biological experts, backed by advanced instrumentation, ensures accurate and reliable results, enabling our clients to make informed decisions and advance their research goals.
- Sample Preparation: Elaborate preparation of biological samples such as blood, urine, tissue or cell lysates, including extraction, purification and concentration. Our expertise in sample preparation ensures removal of potential contaminants and enrichment for biomarkers of interest.
- Chromatographic System Setup: Analysts carefully select appropriate LC columns, mobile phases, and detection methods according to the specific requirements of biomarker analysis. Optimize parameters such as column chemistry, flow rate, gradient profile, and column temperature for optimal separation and detection.
- Sample Injection: Inject the prepared sample into the chromatography system. Ensure accurate and reproducible injections to minimize variability and improve data quality.
- Chromatographic Separation: Injected samples are separated as they pass through the LC column. This separation is based on differential interactions of biomarkers and other sample components with the stationary phase. Our chromatographic conditions are carefully controlled for optimal separation and resolution.
- Detection and Quantification: The separated biomarkers are eluted from the LC column and detected by suitable detectors, such as UV-Vis absorption or mass spectrometry. We ensure accurate quantification of biomarkers, even at low concentrations.
- Data Analysis: Our bioinformatics team processes the acquired chromatographic data using advanced software tools. Perform peak integration, concentration calculations, and statistical analysis to gain meaningful insights and facilitate data interpretation.
Workflow of LC Service
Our Advantages
- Our liquid chromatography methods are carefully optimized to provide excellent sensitivity and increase the accuracy of your analysis.
- A variety of detection techniques compatible with liquid chromatography are available, including UV-Vis absorption, fluorescence, mass spectrometry, and electrochemical detection.
- Advanced sample preparation techniques, including solid-phase extraction, liquid-liquid extraction, and protein precipitation, are employed to ensure optimal extraction, purification, and concentration of biomarkers from complex biological matrices.
- Our bioinformatics team is proficient in data processing, chromatographic integration and statistical analysis to provide clients with comprehensive reports.
Creative BioMart Biomarker provides cutting-edge biomarker analysis solutions. Our high sensitivity, versatility in analyte detection, comprehensive sample preparation and powerful data analysis capabilities enable us to provide accurate and reliable results. If you are interested in our LC service, please feel free to contact us for more information.
