Glycan Labeling and Profiling

Glycans are complex sugar molecules that play crucial roles in many biological processes and are increasingly recognized as important biomarkers for various diseases. Glycan labeling and profiling enables the identification and quantification of glycans in complex biological samples. At Creative BioMart Biomarker, we provide glycan labeling and profiling services to advance biomarker discovery and advance precision medicine.

Application of Glycan Labeling and Profiling in Biomarker Research

Glycan labeling involves the attachment of chemical or fluorescent tags to glycans, facilitating their detection and analysis using a variety of analytical techniques. This process allowed the researchers to unravel the complex structure and composition of glycans. Glycan profiling, on the other hand, refers to the comprehensive analysis of the glycan profile in a given sample. By characterizing glycan profiles, researchers can discover potential biomarkers to elucidate disease mechanisms, diagnosis, prognosis, and treatment response.

Glycan labeling and profiling provide valuable insights into disease progression and treatment response. Glycan profiling in biological samples plays an important role in various fields, including cancer research, autoimmune diseases, and infectious diseases, for example, specific glycan patterns are associated with different cancer types, allowing for early detection and accurate diagnosis.

Glycan Labeling and Profiling Services Offered by Creative BioMart Biomarker

At Creative BioMart Biomarker, we provide high-quality glycan labeling and profiling services to accelerate biomarker discovery. We provide customers with one-stop glycan labeling and profiling services from sample preparation, glycan labeling, purification, analysis to data interpretation.

• Sample Preparation: Collect and process biological samples such as serum, plasma, or tissue extracts to isolate the glycans of interest. This step involves the removal of interfering molecules, including proteins and lipids, to focus on the glycan components.
• Glycan Labeling: Label isolated glycans using advanced labeling methods such as reductive amination or enzymatic labeling. These methods attach chemical or fluorescent tags to glycans for subsequent detection and analysis.
• Purification: After labeling, the labeled glycans undergo a purification step to remove excess labeling reagents and unreacted molecules. This purification process ensures the integrity and purity of the labeled glycan samples.
• Analytical Analysis: Purified and labeled glycans are analyzed using advanced analytical techniques such as high performance liquid chromatography (HPLC), mass spectrometry (MS) and lectin microarrays. HPLC separates the labeled glycans based on their physicochemical properties, while MS provides detailed structural information and quantification.
• Data Interpretation: The generated data are processed and analyzed using bioinformatics tools. Glycan profiles were compared to a reference database and statistically analyzed to identify significant changes and potential biomarkers.

Our Advantages

• We employ two widely recognized analytical techniques, HPLC and MS, to analyze labeled glycans with exceptional sensitivity and resolution. These techniques allow for the detailed characterization and quantification of complex glycan structures.
• Tailor our glycan labeling and profiling services to specific research goals. We consider sample type, experimental design, and desired outcomes to deliver personalized and insightful results.
• Specific glycan structures and potential biomarkers can be identified by comparing the obtained glycan profiles with reference databases.

Workflow of Glycan Labeling and Profiling Service

At Creative BioMart Biomarker, we offer comprehensive glycan labeling and profiling services, utilizing advanced analytical techniques and the power of custom methods. Contact us today to unlock the potential of glycan labeling and analysis in your biomarker research.