Crosslinking Immunoprecipitation (CLIP) Service
In the ever-growing field of molecular biology, the exploration of protein interactions and biomarkers remains at the forefront of research. Cross-linked immunoprecipitation (CLIP) has emerged as a powerful technique to identify and analyze protein-RNA interactions in the cellular environment. Creative BioMart Biomarker has many years of industry experience and is able to provide customers with excellent CLIP services.
What is CLIP?
CLIP is a method used to study the interactions between proteins and their associated nucleic acids in cells. The RNA is covalently linked to the protein by ultraviolet light (UV), thereby preserving transient interactions that might be lost during traditional experimental procedures. Cell lysates containing the cross-linked complexes are then immunoprecipitated using antibodies specific for the protein of interest. Purified protein-RNA complexes were subsequently analyzed.
Application of CLIP in Biomarker Research
By capturing interactions between known biomolecules and previously uncharacterized RNAs, CLIP facilitates the identification of novel biomarker candidates. Biomarker studies often generate a large number of candidates, not all of which are relevant or reliable. CLIP offers a solution by validating the interaction between potential biomarkers and their binding partners. Furthermore, CLIP provides a comprehensive view of protein-RNA networks involved in various diseases, providing important insights into disease mechanisms and potential therapeutic targets.
CLIP Service Offered by Creative BioMart Biomarker
As a leading company in the field of biotechnology, Creative BioMart Biomarker provides efficient and reliable CLIP services for biomarker research. With a professional expert team, cutting-edge technology and rich experience, we are capable of dealing with challenging biomarker research projects.
Our CLIP process begins with careful sample preparation to ensure that the cells or tissues under study are in optimal condition for subsequent cross-linking steps.
By precisely controlling crosslinking conditions, we ensure efficient capture of protein-RNA complexes.
Our group employs antibody-based immunoprecipitation techniques to selectively enrich for proteins of interest and their associated interacting molecules.
- Purification and Sequencing
After immunoprecipitation, we proceed to purify RNA fragments or interacting proteins. The purified complexes are then analyzed using high-throughput sequencing or mass spectrometry.
- Data Analysis and Interpretation
Our expertise in data analysis allows us to discover important interactions and draw meaningful conclusions from the results.
Our CLIP services cover a wide range of applications including but not limited to:
- Biomarker discovery through protein-RNA interaction analysis.
- Validate candidate biomarkers to identify the most promising targets for further development.
- Elucidate disease mechanisms and provide deeper insights into the molecular basis of various diseases.
- Assess treatment response and identify potential resistance mechanisms.
With our CLIP service, researchers have access to precise and reliable data, enabling them to make informed decisions in their biomarker studies.
Why Choose Creative BioMart Biomarker for CLIP Service?
- Have a team of skilled biological experts and technicians to ensure accurate and reliable results.
- Utilize the latest equipment and methods to perform CLIP experiments with exceptional precision and efficiency.
- Provide tailor-made solutions to meet customers' specific requirements.
- In-depth analysis and interpretation of the results, providing a comprehensive analysis report.
Workflow of CLIP Service
As a leading company in the field of biomarkers, Creative BioMart Biomarker provides efficient and high-quality CLIP services, providing researchers with a reliable and cutting-edge platform to advance biomarker research and improve patient care. If you are interested in our services, please feel free to contact us.
