Co-immunoprecipitation (Co-IP) Service
Co-immunoprecipitation (Co-IP) is a powerful technique used in molecular biology and proteomics to study protein-protein interactions and their impact in various cellular processes. In the field of biomarker research, co-IP plays a crucial role in identifying potential biomarkers, validating their interactions, and elucidating their involvement with disease pathways. As a leading expert in the biological industry with many years of experience, Creative BioMart Biomarker has been at the forefront of providing cutting-edge co-IP services for biomarker research.
What is Co-IP?
Co-IP is a technique that enables the selective separation and characterization of protein complexes formed by direct or indirect interactions between proteins in biological samples. This approach exploits the specificity of antibodies to target proteins of interest and their interaction partners, enabling the study of protein networks and signaling pathways.
Figure 1. Schematic diagram of principle of co-IP. (Lin, J.S.; et al. 2017)
Application of Co-IP in Biomarker Research
Co-IP has become an important tool for biomarker identification and validation. By comparing co-IP results between diseased and healthy samples, researchers can identify potential biomarkers based on their interactions with other proteins. Once candidates have been identified, co-IP can be employed to validate their interactions, providing additional evidence of their involvement in disease pathways. Co-IP enables researchers to unravel these complex pathways by identifying protein complexes and their interactions, revealing how biomarkers contribute to disease development.
Co-IP Services Offered by Creative BioMart Biomarker
At Creative BioMart Biomarker, we offer co-IP services tailored specifically for biomarker research. The co-IP process involves several critical steps, including sample preparation, antibody selection, antibody conjugation, immunoprecipitation, washing, elution, and analysis.
We will carefully process customer-supplied cell or tissue samples to extract proteins of interest while maintaining their native conformation and protein-protein interactions.
- Antibody Selection and Conjugation
Use validated antibodies for precise and reliable separation of protein complexes. Our team selects antibodies with high affinity and specificity for the target protein and conjugates them to a solid support such as Protein A/G beads. The antibody-bead complexes act as "baits" to selectively capture the target protein and its interacting partners.
The antibody-bead complexes are incubated with the sample to specifically bind the target protein and its interacting partners.
Following incubation we perform stringent washing steps to remove non-specifically bound proteins and contaminants. Protein complexes are then eluted for further analysis.
We analyze the eluted proteins using techniques such as SDS-PAGE, western blotting or mass spectrometry (MS) and provide customers with comprehensive reports.
Our Advantages
- Carefully select high-affinity and specific antibodies against the target protein to ensure the success of co-IP experiments.
- Extensive sample preparation experience to preserve native conformation and protein-protein interactions.
- Advanced mass spectrometry and proteomics platforms to accurately identify and quantify proteins in isolated complexes.
- Strict quality control measures are adhered to throughout the co-IP process to ensure reproducible results.
Workflow of Co-IP Service
Creative BioMart Biomarker's expertise, advanced technology, and stringent quality control ensure accurate and reliable co-IP results that advance customers' understanding of disease mechanisms. Please feel free to contact us for more service information.
Reference:
- Lin, J.S., Lai, E.M. Protein-Protein Interactions: Co-Immunoprecipitation. Methods Mol Biol. 2017, 1615: 211-219.
