Ascorbic acid is a vitamin present in the human body, and its concentration in plasma and tissues is correlated with status of various diseases, such as scurvy, diabetes mellitus and myocardial infarction. Creative BioMart Biomarker offers high quality detection service for vitamin C, ensuring high detection accuracy, sensitivity and efficiency for each sample.
Vitamin C, which is also known as ascorbic acid (AA), is an indispensable nutrient for human. Vitamin C is a water-soluble vitamin derived mainly from fresh fruit, vegetables and meat. Most animals can synthesize vitamin C by themselves and accumulate in the liver. Vitamin C is an electron donor or reducing agent, and almost all of its physiological effects are related to its electronically involved reactions. Vitamin C is transported by sodium-dependent vitamin C transporters (SVCTs). Among them, SVCT1 is mainly expressed in liver tissue and absorptive tissues in the kidney, including the intestinal epithelium and the proximal convoluted tubules. SVCT2 and glucose transporters (GLUTs) are expressed in most tissues. Vitamin C is a cofactor for various enzymes in the body and also acts as a cosubstrate in the enzyme reactions, so it is significantly associated with a large number of enzyme reactions in the body. For example, 4-hydroxyphenylpyruvate dioxygenase, which is associated with vitamin C, is required in tyrosine catabolism, so vitamin C deficiency causes impaired tyrosine catabolism and increased tyrosine concentration in plasma. Vitamin C is also associated with collagen hydroxylation in the body. Collagen provides structural strength to connective tissues, and vitamin C catalyzes the enzymatic post-translational modification of collagen, allowing collagen-producing cells to produce and secrete enough normal collagen. Therefore, connective tissue often has defects in the patients with scurvy, such as wound cracking, poor wound healing and loose teeth. In addition, vitamin C can play a role in the hydroxylation reaction of hypoxia-inducible factor-1 alpha (HIF-1α) by prolyl 4-hydroxylase. HIF-1 is a transcription factor which is essential for oxygen sensing mechanism in multicellular animals. HIF-1 plays a critical role in normal physiology. For example, it is the key to controlling erythropoiesis and is related to lung diseases, heart disease, and cancer. In addition to scurvy, vitamin C concentration in plasma or tissues may change in many diseases, such as diabetes mellitus, myocardial infarction, acute pancreatitis, and sepsis.
Figure 1. Vitamin C biosynthesis (Figueroa-Méndez, et al. 2015)
Creative BioMart Biomarker strictly controls each specific experimental step in the vitamin C detection procedure to ensure accurately quantify the level of vitamin C in each sample.
At Creative BioMart Biomarker, we offer vitamin C detection service which includes several technical methods, you can communicate with our experts according to your research needs, and we will determine the final detection technological scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.