TRACP-5b Detection Service
TRACP-5b is a biochemical marker of osteoclast activity and number. Its serum level reflects the degree of osteolytic bone metastasis and also reflects the tumor burden in the bone environment. Therefore, serum TRACP-5b level is a biomarker of bone resorption and bears clinical applicability in the diagnosis and treatment of metabolic and pathological bone diseases. Creative BioMart Biomarker is capable to provide our customers with superior TRACP-5B detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.
Introduction
Tartrate-resistant acid phosphatase (TRACP) is a member of the diverse lysosomal enzyme group and has two forms in the circulation: TRACP-5a and TRACP-5b. TRACP-5b is derived from osteoclasts. During bone resorption, osteoclasts secrete TRACP-5b, which produces reactive oxygen species to digest bone degradation products of the bone matrix in the microenvironment. Therefore, it is used as a marker of the activity and numbers of osteoclasts. When TRACP-5b is released into the circulation, it is hydrolyzed by proteases to form fragments that are excreted in the urine and metabolized in the liver. TRACP-5b has several characteristics: i) it is specific for osteoclast number and activity; ii) it is associated with traditional bone resorption collagen markers; iii) it responds rapidly and significantly to anti-resorptive therapy; iv) it has a prognostic value for predicting an increased risk of bone mineral density (BMD) and fracture risk. In addition, TRACP-5b has some advantages in analysis and pre-analysis, exceeding many collagen markers. i) Serum TRACP-5b activity has a lower CVa and CVi because of its small diurnal variation. Compared with NTX and CTX, TRACP-5b is almost unaffected by meals; ii) TRACP-5b is not affected by kidney and liver diseases. Unlike collagen markers, they accumulate at high levels when kidney function or liver function is impaired.
Figure 1. The role of TRACP-5b in osteoclast.
Application of TRACP-5b
- Serum, urine TRACP-5b levels as biomarkers to predict different diseases, such as hyperparathyroidism, osteoporosis and Paget's disease.
Our Advantages
- Accept a wide range of sample types (serum, urine)
- Provide multiple TRACP-5b detection methods, including ELISA, immunoassays
- Ensure high sensitivity for detecting TRACP-5b in different samples
- Ensure high accuracy and repeatable TRACP-5b detection
- Ensure a wide kinetic range to detect samples of different concentrations Short experimental period
Workflow of TRACP-5b Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the TRACP-5b detection procedure to ensure high sensitivity, high accuracy and repeatable TRACP-5b detection.
Please feel free to contact us if you would like to know more about TRACP-5b detection. At Creative BioMart Biomarker, we not only provide high-quality TRACP-5b detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
References:
- Hlaing, T.T.; Compston, J.E. Biochemical markers of bone turnover – uses and limitations. Annals of Clinical Biochemistry. 2014, 51(2): 189-202.
- Chao, T-Y.; et al. Tartrate-resistant acid phosphatase isoform 5b (TRACP 5b) as a serum maker for cancer with bone metastasis. Clinica Chimica Acta. 2010, 411: 1553-1564.
- Preussner, R.; et al. Tartrate-resistant acid phosphatase 5b as a diagnostic marker of bone metastases in patients with renal cell carcinoma. Integr Cancer Sci Therap. 2014, 1(3): 35-38.
