Surfactant Protein A Detection Service
Pulmonary surfactant is a complex of lipids and proteins whose function is to prevent the alveoli from collapsing at expiration. Surfactant A (SP-A) belong to the collectin family and play a key role in the innate immunity of the lung. Since SP-A is abundantly expressed in the lung and only in the lung, the proteins are now used clinically as biomarkers for lung diseases. Creative BioMartBiomarker is capable to provide our customers with superior surfactant protein A detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations. Our SP-A testing service is not suitable for private medical diagnosis and testing.
Surfactant Protein A
Pulmonary surfactant is produced by type II alveolar cells and secreted into the alveolar space, covers all surfaces of 300 million alveoli. In the lung, surfactant protein A (SP-A), B (SP-B), and D (SP-D) are produced in type II alveolar cells and Clara cells. Surfactant protein C (SP-C) is expressed only in type II alveolar cells. SP-A belongs to the collectin subgroup of the C-type lectin superfamily. The structure of SP-A contains four domains, one amino terminus containing a cysteine involved in the formation of interchain disulfide bonds, one collagen-like domain consisting of Gly-X-Y repeats, an amphipathic helix and a lectin domain (a carbohydrate recognition domain (CRD) and a neck domain congaing a short hydrophobic stretch of amino acids. The collagenous domain promotes trimerization, and the trimer undergoes disulfide cross-linking to form higher order oligomers. The levels of SP-A in bronchoalveolar lavage fluids, tracheal aspirates, amniotic fluids, and pleural effusions reflect changes in the alveolar compartment and epithelium, as well as lung maturity. The determination of SP-A in serum can be used to understand some pathological changes of lung diseases, including pulmonary fibrosis, collagen vascular diseases complicated by interstitial lung disease, acute respiratory distress syndrome, alveolar proteinosis and radiation pneumonitis.
Figure 1. Schematic of gene map, transcription and translational products and final protein structure of SP-A (Vieira, et al. 2017)
Application of SP-A
SP-A serum and plasma levels can be used as a biomarker of IPF.
Our Advantages
- Guarantee high accuracy and sensitivity for SP-A detection
- Ensure high repeatability of SP-A detection
- Experienced biomarker detection technical team
- Strictly control each specific experimental step to ensure accurate and reliable test results in each sample
- Short turnaround time to accelerate your research
- More favorable prices in the biomarker testing service market can help reduce research costs
Detection Technologies
Samples
- Sample types: serum, plasma, cell culture supernatants, tissue homogenates, etc.
- Target species: human, mouse, rabbit, rat, canine, etc.
Workflow of SP-A Detection at Creative BioMart Biomarker
Please feel free to contact us if you would like to know more about SP-A detection. At Creative BioMart Biomarker, we not only provide high-quality SP-A detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
References:
- Ballester, B.; et al. Mucins as a New Frontier in Pulmonary Fibrosis. J Clin Med. 2019, 8: 1447.
- Takahashi, H.; et al. Pulmonary surfactant proteins A and D: innate immune functions and biomarkers for lung diseases. Curr Pharm Des. 2006, 12(5): 589-598.
- Vieira, F.; et al. Structure, genetics and function of the pulmonary associated surfactant proteins A and D: the extra-pulmonary role of these C type lectins. Annals of Anatomy - Anatomischer Anzeiger. 2017, 211: 184-201.
