C5b-9 is a complex that exists in membrane-bound form and can reflect the activity of the terminal pathway in the complement system. C5b-9 in plasma is often measured in its soluble form, sC5b-9, and it is also a potential biomarker in the research of diseases such as atypical hemolytic uremic syndrome and diabetic nephropathy (DN). Creative BioMart Biomarker has service experience for many years, and can provide customers with sensitive and specific sC5b-9 testing service. Accurate and reliable results, short turnaround time and reasonable prices can effectively help customers shorten the research cycle and reduce research costs.
The terminal complement complex (TCC, sC5b-9), also known as the membrane attack complex (MAC), is an essential complex for the complement activation pathway. Complement activation can generally be initiated through three different pathways, including the classical pathway (CP), the alternative pathway (AP) and the lectin pathway (LP). Each pathway has a different activation mechanism, and different components participate in the formation of C3 convertase and C5 convertase. C5 convertase can cleave C5 into C5a and C5b, thereby initiating the terminal pathway and assembly of MAC, and finally causing bacterial lysis. In the terminal pathway, C5b formed by cleavage of C5 reacts with C6 and C7 molecules to form C5bC6C7, resulting in a hydrophobic mC5b-7 complex. The mC5b-7 complex binds to C8 molecules, followed by a membrane insertion event. 12-18 copies of C9 molecules polymerize and form the lytic pore (C5b-9) with C5b-8. This transmembrane channel can induce microbial membrane lysis by affecting the balance of cell osmotic pressure, leading to cell death. The change in the level of sC5b-9 is related to the activity of the terminal pathway, so it can be used to monitor its activity. The complement system is involved in a variety of diseases. Complement deficiency and excessive complement activation are all related to the pathogenesis of inflammatory diseases. Therefore, sC5b-9 is often used as a biomarker in the research of inflammatory diseases, such as thrombotic microangiopathy (TMA) and complement 3 glomerulopathy (C3G).
Figure 1. MAC formation and the resultant consequences in target cell (Andrade, et al. 2017)
sC5b-9 levels can be used as biomarkers in researches related to atypical hemolytic uremic syndrome, DN, TMA, C3G, etc.
Creative BioMart Biomarker strictly controls each specific experimental step in the detection procedure to ensure accurately quantify the level of sC5b-9 in each sample.
At Creative BioMart Biomarker, we offer high-quality sC5b-9 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.