PYD is derived from the degradation of collagen and is of interest as a biomarker for bone resorption for assessing the risk of fracture in people with osteoporosis, bone cancer and other collagen diseases. In addition to the monomeric PYD, Glc-Gal-PYD 4 also can be used as a biomarker to monitor patients who suffer from various joint diseases or synovium degradation. Creative BioMart Biomarker is capable to provide our customers with superior PYD detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.
Introduction
Pyridinoline (PYD) is a molecule that mechanically stabilizes collagen by crosslinking single collagen peptides. Collagen cross-linking is the major post-translational modification of collagen and plays an important role in the biological and biomechanical properties of bone. Collagen cross-linking can be divided into lysyl hydroxylase and lysyl oxidase-mediated enzymatic immature bivalent cross-linking, mature trivalent pyridinoline and pyrrole cross-linking, and non-enzymatic induction by saccharification or oxidation promote cross-linking such as glucosepane, pentosidine, etc. PYD is mainly found in cartilage, bones, ligaments and blood vessels. Collagen cross-linking is formed during the extracellular maturation of collagen and is released from the degradation of mature collagen into the circulation. PYD is released into the urine either freely (40%) or in combination with the polypeptide (60%), and most of the PYD released into the urine is derived from the bone. The level of urine PYD in patients with osteoporosis or other metabolic bone diseases is significantly higher than in healthy people. In addition to the monomeric PYD, Glc-Gal-PYD 4, a diglycosylated PYD, can represent a synovium status, as a biomarker to monitor patients suffering from various joint diseases or synovium degradation.
Figure 1. Chemical structure of PYD, Gal-PYD, Glc-Gal-PYD 4.
Application of PYD
- Serum, urine free/total PYD levels as biomarkers to predict different diseases, such as osteoporosis, bone cancer and other collagen diseases.
Our Advantages
- Accept a wide range of sample types (serum, urine)
- Provide multiple PYD detection methods, including ELISA, HPLC
- Ensure high sensitivity for detecting PYD in different samples
- Ensure high accuracy and repeatable PYD detection
- Ensure a wide kinetic range to detect samples of different concentrations
- Short experimental period
Workflow of PYD Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the PYD detection procedure to ensure high sensitivity, high accuracy and repeatable PYD detection.
Please feel free to contact us if you would like to know more about PYD detection. At Creative BioMart Biomarker, we not only provide high-quality PYD detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
References:
- Hlaing,T.T.; Compston, J.E. Biochemical markers of bone turnover – uses and limitations. Annals of Clinical Biochemistry. 2014, 51(2):189-202.
- Anastasia,L.; et al. Chemical structure, biosynthesis and synthesis of free and glycosylated pyridinolines formed by cross-link of bone and synovium collagen. Org. Biomol. Chem. 2013, 11: 5747-5771.
