Human serum paraoxonase-1 (PON1) is a protein associated with the function of high-density lipoprotein (HDL) and with cardiovascular diseases. Creative BioMart Biomarker provides customers engaged in biomarker research with fast, sensitive and accurate PON1 detection services, which can help customers quickly analyze the concentration and activity of PON1. Our PON1 testing service is only for research use, not for personal diagnosis and testing.
Our Services for PON1 Detection
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PON1 Concentration Measurement |
We use fluorometric method to quickly measure the activity of PON1. |
| PON1 Activity Measurement |
Creative BioMart Biomarker uses enzyme-linked immunosorbent assay to measure the concentration of PON1 in the sample. |
Sample Type: serum, plasma, cell culture supernatants, tissue homogenate or other biological fluids.
Biomarker Application Area: cardiovascular diseases, liver disease.
* Perform three replicate tests on each sample, and include appropriate controls in each assay.
Assay Background
PON1 belongs to the paraoxonase family, which includes PON2 and PON3 in addition to PON1. PON1 is a calcium-dependent hydrolase related to HDL function. PON1 is mainly produced in the liver and secreted into the blood. It is composed of 354 amino acids, has a molecular weight of 43 kDa, and has a six-blade β-propeller structure with a central tunnel that can accommodate two calcium ions. One calcium ion is essential for the conformational stability of PON1, and the other calcium ion is important for substrate positioning and ester bond activation. Therefore, calcium ions play an important role in the activity of PON1. PON1 has a variety of hydrolytic activities, including lactonase activity, arylesterase activity and organophosphatase activity, and also has homocysteine thiolactonase activity, which can prevent N-homocysteinylation. PON1 plays a key role in reducing the accumulation of lipid peroxides on low-density lipoproteins (LDL). PON1 has the ability to hydrolyze oxidized LDL-cholesterol and has an atheroprotective effect. At the same time, it can lyse phospholipid peroxidation adducts, which has potential cell protection function. Therefore, PON1 is often used in atherosclerotic cardiovascular disease research.
Workflow of Biomarker Detection Services at Creative BioMart Biomarker
Customers provide: samples, reagents/kits (reagents/kits can also be provided or purchased by our company).
We provide: remaining samples and reagents, complete experimental reports (including experimental raw data, experimental procedures, instrument and reagent information, and professional result analysis).
Test cycle: 10 working days or less.
Why Choose Creative BioMart Biomarker for PON1 Detection Service?
- High-quality ELISA technology platform
Over the years, our expert team has spent a lot of energy to establish a high-quality ELISA technology platform, and select high sensitivity and specificity of ELISA kits for testing to meet the experimental needs of customers.
- Experienced experts team
Our experts have extensive experience in biomarker testing, and will perform each test in full accordance with standard experimental operating procedures to ensure that the test results delivered are accurate and repeatable.
- Suitable for multiple sample types
Our biomarker testing service is applicable to several sample types, including serum, plasma, cell culture supernatant, tissue homogenate, etc. If your sample type is not common, you can contact us at any time to inquire about the testing service for your sample.
- Fast testing and reasonable price
We have been committed to providing customers with efficient services to ensure that the experimental results are delivered within the predetermined time. Our testing services have reasonable and competitive prices in the industry, which can help customers reduce research costs.
If you are interested in our services, please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:
- Shunmoogam, N.; et al. Paraoxonase (PON)-1: a brief overview on genetics, structure, polymorphisms and clinical relevance. Vascular Health & Risk Management. 2018, 14: 137-143.
- Kowalska, K.; et al. Review: the role of paraoxonase in cardiovascular diseases. Annals of Clinical & Laboratory Science. 2015, 45(2): 226-233.
