PINP is a reliable biomarker of bone turnover and is used to monitor bone formation. PINP levels in serum have been measured to assess bone turnover, detect diseases and monitor therapy in different diseases, such as Paget's disease, osteoporosis, van Buchem disease, rheumatoid arthritis. Creative BioMart Biomarker is capable to provide our customers with superior PINP detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.
Introduction
Procollagen type I N-terminal propeptide (PINP) is derived from type I procollagen. Procollagen consists of a triple helical molecule which contains two α1 (I) chains and one α2 (I) chain. The triple helix is flanked by nonhelical telopeptides, N-terminal propeptides (PINP) and C-terminal propeptides (PICP). After cleaved by specific endoproteases, it released type I collagen and circulating PINP and PICP. The circulating PINP concentration is proportional to the amount of new collagen matrix being formed and subsequently new mineralized bone. Therefore, PINP is a serum biomarker associated with bone formation. PINP has a high sensitivity, furthermore, it is less sensitive to variations during the day and after food intake. Therefore, PINP concentration is a sensitive biomarker to monitor therapy in Paget's disease, osteoporosis, van Buchem disease, rheumatoid arthritis, and so on. Trimeric PINP is a good marker for follow up of disease activity in mono-ostotic disease or in case of abnormal kidney function. Especially in osteoporosis, PINP is the marker of choice to monitor therapy. In addition, studies in rat have shown that PINP as a bone formation biomarker in rodents, could be a useful tool for preclinical evaluation of treatments.
Figure 1. Structure of PINP.
Application of PINP
- PINP level in serum have been measured to assess bone turnover, detect diseases and monitor therapy in different diseases, such as Paget's disease, osteoporosis, van Buchem disease, rheumatoid arthritis.
Our Advantages
- Accept a wide range of sample types (serum, plasma, tissue homogenates, cell culture supernates etc.)
- Provide multiple PINP detection methods, including ELISA, ECL and RIA
- Ensure high sensitivity for detecting PINP in different samples
- Ensure high accuracy and repeatable PINP detection
- Ensure a wide kinetic range to detect samples of different concentrations
- Short experimental period
Workflow of PINP Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the PINP detection procedure to ensure high sensitivity, high accuracy and repeatable PINP detection.
Please feel free to contact us if you would like to know more about PINP detection. At Creative BioMart Biomarker, we not only provide high sensitivity, high accuracy and repeatable PINP detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
References:
- Hale, L.V.; et al. PINP: A serum biomarker of bone formation in the rat. Bone. 2007, 40:1103-1109.
- Vlota, M.C.; et al. Clinical utility of bone markers in various diseases. Bone. 2018, 114:215-225.
