N-Terminal propeptide of type III collagen (PIIINP) is a derivative produced during the synthetic process of type III collagen that is associated with osteoarthritis (OA), rheumatoid arthritis (RA), and synovitis especially, therefore it is often used as a biomarker for arthritis. Creative BioMart Biomarker offers high quality detection service for PIIINP, ensuring high detection accuracy, sensitivity and efficiency in each sample.
Introduction
PIIINP is a homotrimer with a total molecular weight of 42 kDa that is served as a serum biomarker for collagen turnover. The PIIINP in serum can be derived from the synthesis process of type III collagen, or from the degradation of existing type III collagen fibrils. In addition to bone and cartilage, the third important tissue compartment in the joint is the synovium. The synovium is a special type of connective tissue that lines the diarthrodial joints, surrounds the tendons and forms the lining of bursae and fat pads. Synovitis refers to a joint lesion in which the synovial membrane is stimulated to produce inflammation, resulting in the imbalance of the secretion to form hydrops. Synovitis is associated with further deterioration of the OA structure and is a common feature of OA. The histological features of the synovium in patients with OA include synovial lining cell hyperplasia, fibrosis, and stromal vascularization. Serum PIIINP can be used to assess synovitis in OA and RA, and it is significantly associated with joint space area (JSA) and minimal joint space width (JSW) of the knee. PIIINP is positively correlated with knee pain, stiffness, and dysfunction, and the serum concentration of which increases with the severity of cartilage damage. Serum PIIINP levels can be used to assess disease activity and joint damage in OA patients. In addition, serum levels of PIIINP are also high in RA patients, and PIIINP is associated with conventional markers of RA disease activity, such as C-reactive protein (CRP) and joint swelling score. Moreover, PIIINP is a stable biomarker with good stability at 4 °C. PIIINP is a very useful biomarker of synovitis, and is also a diagnostic and prognostic biomarker of OA and RA that has development prospects in the research of arthritis diseases.
Figure 1. Structural domains of type III procollagen molecule. (Kuivaniemi, et al. 2019)
Application of PIIINP Detection
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Serum PIIINP levels as biomarkers to predict different diseases, such as rheumatoid arthritis, osteoarthritis, synovitis, etc.
Our Advantages
- Guarantee high accuracy and sensitivity for PIIINP detection
- Ensure high repeatability of PIIINP detection
- Short turn-around time of detection service
- Competitive price in the market of detection services
- Provide multiple PIIINP detection methods, including ELISA and RIA
- Accept a wide range of sample types (serum, plasma, etc.)
Workflow of PIIINP Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the PIIINP detection procedure to ensure accurately quantify the level of PIIINP in each sample.
At Creative BioMart Biomarker, we offer PIIINP detection service that includes several technical methods, you can communicate with our experts according to your research needs, and we will determine the final detection technological scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:
- Ehlers, M.R.; Leary, E.T. (2008) Biochemical markers of rheumatoid arthritis and osteoarthritis: clinical utility and practical considerations. Reid, D.M.; Miller, C.G. (Eds.), Clinical Trials in Rheumatoid Arthritis and Osteoarthritis (pp. 151-169). London: Springer-Verlag London Limited.
- Garnero, P.; et al. Cross sectional evaluation of biochemical markers of bone, cartilage, and synovial tissue metabolism in patients with knee osteoarthritis: relations with disease activity and joint damage. Annals of the Rheumatic Diseases. 2001, 60(6): 619-626.
- Kuivaniemi, H.; Tromp, G. Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. 2019, 707: 151-171.
