Carboxyl-terminal propeptide of type I collagen (PICP or CICP) is a major secretory protein of osteoblasts which play a critical role in bone formation process, therefore PICP is served as a biomarker for bone disease and bone formation mechanism. Creative BioMart Biomarker offers high quality detection service for PICP, ensuring high detection accuracy and efficiency for each sample.
Introduction
PICP is an extension of type I collagen carboxyl-terminal polypeptide that is released into blood circulation after the maturation and rupture of type I procollagen. Type I collagen is the most abundant collagen in human body, which is an important component of bones, and it is accounting for about 90% of the organic part of bone matrix, so it is important to analyze the synthesis of type I collagen for bone formation monitoring. Type I collagen is derived from procollagen which is its precursor molecule. Procollagen is a rod-like triple helix molecule that with a spherical amino (N) - and carboxyl (C) - terminal propeptide domain. Type I collagen is a heterotrimer that is composed of two α1 chains and one α2 chain. In the process of expression and secretion for procollagen cells, it is first assembled in the form of a trimer, and then cleavage at a specific N-terminal and C-terminal site by the action of a specific enzyme to produce an amino-terminal propeptide of type I collagen (PINP), type I collagen, and PICP. When matured type I collagen is polymerized into extracellular collagen fibers, PINP and PICP are released into the blood. Type I collagen is a protein that is ubiquitous in the human body, not bone-specific. However, type I collagen accounts for a large percent of bone matrix proteins, in addition, the weight of bone is higher than other tissues that contain type I collagen, and the metabolic rates of bone are also faster than these tissues. Therefore, the levels of PINP and PICP in serum are significantly correlated with bone formation in bone histomorphometry. PICP as a biomarker can represent the formation of type I collagen in bone, besides, serum levels of PICP have been used as indicators of bone formation and metabolism in studying the physiological conditions and diseases associated with bone formation.
Figure 1. Schematic diagram showing the generation of PICP from type I procollagen by enzymatic cleavage (Seo, et al. 2017)
Application of PICP Detection
- Plasma and serum PICP levels as biomarkers to predict different diseases, such as Paget’s disease, hyperthyroidism, primary hyperparathyroidism, renal osteodystrophy, etc.
Our Advantages
- Guarantee high accuracy and sensitivity for PICP detection
- Ensure high repeatability of PICP detection
- Short turn-around time of detection service
- Competitive price in the market of detection services
- Provide multiple PICP detection methods, including ELISA and RIA
- Accept a wide range of sample types (serum and plasma, etc.)
Workflow of PICP Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the PICP detection procedure to ensure accurately quantify the level of PICP in each sample.
At Creative BioMart Biomarker, we offer PICP detection service that includes a variety of technical methods, you can communicate with our experts according to your research needs, and we will determine the final detection technological scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
Reference:
- Seo, W. Y.; et al. Production of recombinant human procollagen type I C-terminal propeptide and establishment of a sandwich ELISA for quantification. Scientific Reports. 2017, 7(1): 15946.
