Prostatic acid phosphatase (PAP) is a protein synthesized by the prostate gland and has been studied as a biomarker of prostate cancer for many years. Creative BioMart Biomarker provides customers with PAP testing services suitable for different sample types and helps customers accelerate relevant biomarker research. This test is only suitable for research use and not for clinical use.
|
PAP Testing |
| Analyte: |
PAP |
| Synonyms: |
prostatic acid phosphatase, prostatic specific acid phosphatase, PSAP |
| Sample Type: |
cell culture supernatant, plasma, serum, tissue homogenate and other biological fluids |
| Assay Platform: |
ELISA, CLIA |
| Biomarker Application Area: |
prostate cancer |
| * Perform three replicate tests on each sample, and include appropriate controls in each assay. |
Assay Background
PAP is a glycoprotein synthesized by lysosomes of prostate epithelial cells with a molecular weight of 100kDa. The expression of PAP is related to testosterone and PAP has two forms, namely in the cellular form (cPAP) and the secretory form (sPAP). Two different forms of PAP have different biochemical properties, including hydrophobicity, glycosylation mode, isoelectric point, etc. cPAP has cellular protein tyrosine phosphatase activity and is highly expressed in prostate cells. Although sPAP is also expressed in prostate, most of it will be released into semen. The mRNA and protein expression levels of cPAP are reduced in prostate cancer tissues. Down-regulation of cPAP expression affects extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) signal transduction pathway, PI3K-AKT-AR pathway, and changes the RANKL/OPG system, in which the activation of ERK/MAPK signal transduction pathway is related to the development of prostate cancer. PAP can be used as a biomarker of prostate cancer, which is of significance for the monitoring and management of prostate cancer.
Workflow of Biomarker Detection at Creative BioMart Biomarker
Customers provide: samples, reagents/kits (reagents/kits can also be provided or purchased by our company).
We provide: remaining samples and reagents, complete experimental reports (including experimental raw data, experimental procedures, instrument and reagent information, and professional result analysis).
Test cycle: 10 working days or less.
Why Choose Creative BioMart Biomarker for PAP Detection Service?
- High-quality technology platform
Creative BioMart Biomarker has established a number of technical platforms that can be used for biomarker detection. We provide ELISA and CLIA technology platforms for PAP testing, and you can choose according to your research needs.
- Experienced experts team
Our experts have extensive experience in biomarker testing, and will perform each test in full accordance with standard experimental operating procedures to ensure that the test results delivered are accurate and repeatable.
- Suitable for multiple sample types
Our biomarker testing service is applicable to several sample types, including cell culture supernatant, plasma, serum, tissue homogenate, etc. If your sample type is not common, you can contact us at any time to inquire about the testing service for your sample type.
- Fast testing and reasonable price
We have been committed to providing customers with efficient services to ensure that the experimental results are delivered within the predetermined time. Our testing services have reasonable and competitive prices in the industry, which can help customers reduce research costs.
If you are interested in our services, please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:
- Xu, H.; et al. Prostatic acid phosphatase (PAP) predicts prostate cancer progress in a population-based study: the renewal of PAP? Disease Markers. 2019, 2019(3): 1-10.
- Kong, H. Y.; Byun, J. Emerging roles of human prostatic acid phosphatase. Biomolecules & Therapeutics. 2013, 21(1): 10-20.
