Tumor protein p53 is a protein with anticancer properties and is closely related to DNA damage repair and apoptosis. In some cancers, p53 expression levels are up-regulated, and this may serve as a biomarker for tumors early diagnosis. Creative BioMart Biomarker provides customers with accurate, sensitive and specific cancer biomarker testing services, including p53 detection services. High-quality services, short turnaround time and reasonable prices can effectively help customers shorten the research cycle and reduce research costs.
p53 and Cancers
p53 is a 53kDa protein identified in tumor cell lines and has anti-cancer properties, so it has been widely studied in various types of cancer. As a DNA sequence-specific transcription factor, p53 plays an important role in cell stress response and is related to cell cycle arrest, DNA damage repair, cell senescence and apoptosis. After DNA damage occurs, cyclin D is degraded, and then p53 promotes the increase of CDKN1A protein expression level, resulting in the formation of cyclin E/CDK2 complex and stopping the cell cycle process by inhibiting pRB phosphorylation. Moreover, CDKN1A may promote DNA repair by binding to PCNA. In addition, after DNA damage occurs, p53 activates signaling pathways related to G2 blockade. p53 is also associated with apoptosis. It can induce apoptosis through multiple pathways and is an effective inducer of apoptosis. Mouse double minute (MDM2) is a protein that can bind to p53 and can act as a ubiquitin ligase to link ubiquitin and p53, thereby promoting p53 degradation by the proteasome. That is, p53 expression level is regulated by MDM2. At the same time, MDM2 is also a transcription target of p53, so an autoregulatory feedback loops is formed between the two. The abnormal level of p53 in tumors may also be related to the upregulation of MDM2, for example, in kidney cancer, prostate cancer and esophageal cancer. Therefore, mutations in the p53 gene or changes in its expression in tumors will cause changes in p53 protein levels, and increased p53 protein levels may be used for early diagnosis of tumors.
Figure 1. p53-MDM2 regulatory feedback loop under unstressed condition and p53 function under stressing stimula (Urso, et al. 2015)
Application of p53 Detection
Plasma and serum p53 levels can be used as biomarkers in researches related to kidney cancer, prostate cancer, esophageal cancer, bladder cancer, etc.
Our Advantages
- Guarantee high accuracy and sensitivity for p53 detection
- Ensure high repeatability of p53 detection
- Short turn-around time of detection service
- Competitive price in the market of p53 detection services
- Multiple technology platforms for p53 test services (ELISA, ECL)
- Accept a wide range of sample types (plasma, serum, cell culture supernatants, etc.)
Workflow of Biomarker Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the detection procedure to ensure accurately quantify the level of p53 in each sample.
At Creative BioMart Biomarker, we offer high-quality p53 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:
- Boyd, M. T.; Vlatkovic, N. P53: a molecular marker for the detection of cancer. Expert Opinion on Medical Diagnostics. 2008, 2(9): 1013-1024.
- Urso, L.; et al. Critical review about MDM2 in cancer: possible role in malignant mesothelioma and implications for treatment. Critical Reviews in Oncology/Hematology. 2015, 97(8428): 220-230.
