Osteopontin (OPN) is involved in many pathological and physiological processes of the body. In addition to bone remodeling, it is also involved in cell adhesion, tumor cell proliferation and tumor metastasis. Creative BioMart Biomarker provides customers with accurate, sensitive and specific cancer biomarker testing services, including osteopontin detection services. High-quality services, short turnaround time and reasonable prices can effectively help customers shorten the research cycle and reduce research costs.
OPN is a highly phosphorylated and glycosylated sialoprotein protein that belongs to the non-collagenous proteins family SIBLING (small integrin-binding ligand, N-linked glycoprotein). OPN is a versatile protein that is not only expressed in osteoclasts and osteoblasts, involved in bone remodeling. It is also an integral part of the immune system, secreted by neutrophils, NK cells, T cells and B cells, and can activate immune cells and regulate the immune system. OPN can communicate with cells through integrins, and can also participate in intracellular interactions through the interaction between its RGD sequence and CD44v 6/7. In addition, OPN is involved in cell differentiation and the regulation of cytokine production. OPN can activate downstream signaling pathways including protein kinase C (PKC) and phosphoatidylinositol 3-kinase (PI3K/Akt) via phospholipase C-γ (PLCγ), and OPN can also activate JNK1 (c-Jun N-terminal kinase 1) signaling pathways through nuclear factor-inducing kinase (NIK) and MEKK1 (mitogen-activated protein kinase kinase kinase1). OPN mediates the progression of various tumors, including breast cancer, prostate cancer, melanoma, etc. Increased OPN secretion is associated with the proliferation of tumor cells. On the other hand, OPN is closely related to the metastasis of tumor cells in diseases such as lung cancer and melanoma. In addition, OPN may also act on tumor angiogenesis and endothelial cell recruitment through activation with vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1 (HIF-1α). The key role of OPN in tumor progression may make it an effective biomarker and anti-cancer therapeutic target.
Figure 1. The role of osteopontin in multi-steps of cancer cell metastasis (Zhao, et al. 2019)
Plasma and serum osteopontin levels can be used as biomarkers in researches related to breast cancer, melanoma, stomach cancer, prostate cancer, etc.
Creative BioMart Biomarker strictly controls each specific experimental step in the detection procedure to ensure accurately quantify the level of osteopontin in each sample.
At Creative BioMart Biomarker, we offer high-quality osteopontin detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.