Brain natriuretic peptide (BNP) and N-terminal proBNP (NT-proBNP) are widely used in clinical medicine as diagnostic biomarkers for heart failure (HF) and cardiac dysfunction. In forensic medicine, they can also be used as post-mortem biomarkers to reflect the heart function of the deceased before death. Creative BioMartBiomarker is capable to provide our customers with superior NT-proBNP detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.
The natriuretic peptide family mainly includes atrial natriuretic peptide (ANP), which mainly synthesizes and secretes by atrial myocytes, BNP and C-type natriuretic peptide (CNP). BNP is mainly synthesized and secreted by myocytes in the left ventricle (LV) as a response to stretching of myocytes due to pressure overload or ventricular volume expansion. The transcription of BNP mRNA and the synthesis and secretion of BNP proteins occur in an explosive manner, and are quickly released into surrounding tissues after myocardial synthesis. Under pathological conditions, unstable mRNA can quickly synthesize a 134 amino acid BNP precursor (pre-proBNP) and remove the 26-amino acid signal peptide at the N-terminus to form a 108-amino acid BNP (proBNP). The proBNP is split by the proNP convertase, corin or furin, into an inactive 76-amino acid NT-proBNP and an active BNP. BNP and NT-proBNP are detectable in plasma.
Figure 1. brain natriuretic peptide (BNP) structural processing.
NT-proBNP and BNP are considered to be the most valuable and reliable biomarkers for diagnosing HF and cardiac dysfunction. They can also measure the severity of the disease, guide related treatment strategies, and assess the prognosis of heart disease. In a non-acute environment, the normal upper limit of NT-proBNP is 125 pg/mL, and in an acute environment, the critical value of NT-proBNP is 300 pg/mL. Studies have shown that the age-dependent cutoff of NT-proBNP may be more effective in the diagnosis of HF. Generally, 300 pg/mL can rule out acute HF. However, NT-proBNP levels> 450 pg/mL under 50 years old, NT-proBNP levels> 900 pg/mL between 50-75 years old and NT-proBNP levels> 1800 pg/mL over 75 can be diagnosed as a patient with HF.
Plasma levels of NT-proBNP, can be used as a potential biomarker for cardiovascular disease.
Creative BioMart Biomarker strictly controls each specific experimental step in the NT-proBNP detection procedure to ensure high sensitivity, high accuracy and repeatable NT-proBNP detection.
Please feel free to contact us if you would like to know more about NT-proBNP detection. At Creative BioMart Biomarker, we not only provide high-quality NT-proBNP detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.