LCAT Detection Service

Lecithin–cholesterol acyltransferase (LCAT) is an enzyme that mediates the formation of cholesteryl ester. The determination of its activity and mass can help explore the pleiotropic effects of high density lipoprotein cholesterol (HDL). Therefore, LCAT is often used as a biomarker of HDL functionality. Creative BioMart Biomarker provides customers with high-quality LCAT analysis tests, which include the determination of its concentration and activity. Our LCAT testing service is not suitable for private medical diagnosis and testing.

Our Services for LCAT Detection

LCAT Concentration Measurement We provide customers with enzyme-linked immunosorbent assay service for the measurement of LCAT concentration.
LCAT Activity Measurement LCAT activity is determined using an exogenous apoA-I proteoliposome substrate.

Sample Type: serum, plasma, tissue homogenate or other biological fluids.

Biomarker Application Area: cardiovascular diseases, such as coronary heart disease, and ovarian cancer.

* Perform three replicate tests on each sample, and include appropriate controls in each assay.

Assay Background

LCAT is a 63kDa enzyme protein mainly synthesized by the liver, which can convert free cholesterol into cholesteryl ester. After LCAT binds to lipoproteins, fatty acids are cleaved from the sn-2 position of phosphatidylcholine and transferred to the 3-β-hydroxyl group on the A-ring of cholesterol to form cholesteryl ester. Since cholesteryl ester has stronger hydrophobicity, it is sequestered into the hydrophobic core of lipoprotein particles. LCAT can promote the efflux of cholesterol from peripheral cells and affect the concentration gradient of free cholesterol between the plasma membrane and HDL particles. At the same time, CETP can affect the ability of HDL to bind cholesterol by transferring the cholesteryl ester formed by LCAT. In addition, LCAT affects the spontaneous back exchange of cholesterol from HDL to cells. The LCAT activity assay can measure the esterification of free cholesterol. At the same time, the measurement of LCAT activity may also help assess cardiovascular risk, and the regulation of LCAT activity is also one of the research directions in the treatment of coronary heart disease.

Workflow of Biomarker Detection at Creative BioMart Biomarker

Workflow

Customers provide: samples, reagents/kits (reagents/kits can also be provided or purchased by our company).

We provide: remaining samples and reagents, complete experimental reports (including experimental raw data, experimental procedures, instrument and reagent information, and professional result analysis).

Test cycle: 10 working days or less.

Why Choose Creative BioMart Biomarker for LCAT Detection Service?

  • High-quality ELISA technology platform
  • Over the years, our expert team has spent a lot of energy to establish a high-quality ELISA technology platform, and select ELISA kits with high sensitivity and specificity for testing to meet the experimental needs of customers.

  • Experienced experts team
  • Our experts have extensive experience in biomarker testing, and will perform each test in full accordance with standard experimental operating procedures to ensure that the test results delivered are accurate and repeatable.

  • Suitable for multiple sample types
  • Our biomarker testing service is applicable to several sample types, including serum, plasma, tissue homogenate, etc. If your sample type is not common, you can contact us at any time to inquire about the testing service for your sample.

  • Fast testing and reasonable price
  • We have been committed to providing customers with efficient services to ensure that the experimental results are delivered within the predetermined time. Our testing services have reasonable and competitive prices in the industry, which can help customers reduce research costs.

If you are interested in our services, please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.

Reference:

  1. Rousset, X.; et al. Lecithin:cholesterol acyltransferase: from biochemistry to role in cardiovascular disease. Current opinion in endocrinology, diabetes, and obesity. 2009, 16(2): 163–171.

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