HMGB1 Detection Service

High mobility group box 1 (HMGB1) is a protein that is highly expressed in various cancers and is associated with tumor invasion, metastasis and angiogenesis, and is a potential biomarker for tumorigenesis and development. Creative BioMart Biomarker has been committed to providing customers with high-quality cancer biomarker test services, including HMGB1 detection services. Our technical team has rich experience and can offer customers test results with high accuracy, sensitivity and repeatability. Fast testing and reliable results can effectively help customers shorten the research cycle.

HMGB1 and Cancers

HMGB1 is a 30kDa non-histone nucleoprotein with two DNA binding cassettes (A-box and B-box). In addition, there is a negatively charged C-terminal tail with a continuous amino acid sequence of glutamic acid and aspartic acid. As a highly conserved protein, it has important functions in vivo. Its post-translational modifications come in many forms, including glycosylation, acetylation, methylation and phosphorylation forms, of which only acetylated HMGB1 can form a specific complex with the homologous DNA polymerase α and activate the enzyme. While the acetylated and phosphorylated forms can cause the transport of HMGB1 from the nucleus to the cytoplasm. HMGB1 is a nuclear protein involved in the regulation of transcription levels of various genes, such as tumor necrosis factor alpha, breast cancer susceptibility genes, etc. HMGB1 in vivo has many different functions, including maintaining the stability of the genome, regulating DNA repair, participating in the regulation of cell autophagy, and promoting the secretion of cytokines. HMGB1 is associated with the occurrence and development of different types of cancer, including liver cancer, breast cancer, prostate cancer, and ovarian cancer. During the growth and development of tumors, tumor cells grow exponentially, and the existing blood vessels cannot supply sufficient nutrients. Therefore, the subsequent chronic hypoxia leads to tumor necrosis and releases HMGB1. On the other hand, HMGB1 can also activate the production of vascular endothelial cells. It can stimulate endothelial cell proliferation through the combination with RAGE, and promote neovascularization. In addition, HMGB1 protein interacts with other transcription factors on the promoter to regulate the expression of cytokine genes, such as p53 and Rel/NF-κB family, thereby promoting the occurrence and development of cancer. At the same time, HMGB1 is also associated with autophagy and death of tumor cells.

Figure 1. Structural arrangement of HMGB1 (Tripathi, et al. 2019)

Application of HMGB1 Detection

Plasma and serum HMGB1 levels can be used as biomarkers in researches related to breast cancer, liver cancer, prostate cancer, ovarian cancer, etc.

Our Advantages

• Guarantee high accuracy and sensitivity for HMGB1 detection
• Ensure high repeatability of HMGB1 detection
• Short turn-around time of detection service
• Competitive price in the market of HMGB1 detection services
• Multiple technology platforms for HMGB1 test services (ELISA, RIA, CLIA)
• Accept a wide range of sample types (plasma, serum, cell culture supernatants, etc.)

Workflow of Biomarker Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the detection procedure to ensure accurately quantify the level of HMGB1 in each sample.