Flow cytometry is a powerful technique used in the field of biomedical research. It allows the analysis of individual cells or particles in heterogeneous populations, providing valuable information about their characteristics, such as cell surface markers, protein expression, and functional status. Creative BioMart Biomarker provides personalized flow cytometry services to help clients advance biomarker research.
Flow Cytometry and Biomarker
Flow cytometry is a technique that combines the principles of fluid dynamics, optics, and cell biology to allow rapid screening and analysis of single cells in solution. In flow cytometry, a sample of cells or particles is stained with a fluorescent dye to make a sample suspension, which is then formed into a single-file stream and intersected with an incident laser beam. The resulting scattered light and fluorescent signals are detected by detectors such as photodiodes or photomultiplier tubes. This scatter and fluorescence emission provide parametric information about the cell, allowing the cell to be characterized.
Flow cytometry enables researchers to analyze biomarkers at the single-cell level, providing valuable insights for understanding disease pathogenesis, monitoring immune responses, and identifying potential therapeutic targets. By utilizing fluorescently labeled antibodies to specific markers, flow cytometry can differentiate between different cell types and subpopulations in heterogeneous samples. In addition, intracellular biomarkers such as phosphorylated proteins or cytokines can be analyzed by flow cytometry.
Flow Cytometry Service
Creative BioMart Biomarker offers comprehensive flow cytometry services for biomarker analysis to meet the needs of research applications. Creative BioMart Biomarker has rich industry experience, can provide customers with accurate and reliable analysis results, and can also customize solutions according to different research situations.
- Sample Preparation and Staining
Standardized and optimized sample preparation is important for flow cytometry analysis. We not only design and implement appropriate staining protocols, but also provide optimized protocols for antibody selection, fluorochrome conjugation, and sample handling to ensure reliable and reproducible results.
We employ advanced flow cytometry that facilitates the measurement of various fluorescence parameters, enabling comprehensive analysis of biomarkers.
- Data Analysis and Interpretation
Flow cytometry generates large data sets that require sophisticated data analysis tools and expertise. Creative BioMart Biomarker provides comprehensive data analysis and interpretation services to extract meaningful insights from flow cytometry data.
We also provide cell sorting services using flow cytometry and ensure high efficiency, viability, and purity of sorted cells for confidence in downstream experiments.
Our Advantages
- The team of skilled scientists and technicians has extensive expertise in experimental design, sample preparation, data analysis and interpretation.
- Continuous investment in new flow cytometry instruments and techniques, resulting in superior sensitivity and resolution.
- A robust quality management system ensures reproducible results.
- According to the specific requirements and goals of our customers, our team of experts can provide customized solutions.
Workflow of Flow Cytometry Service
Creative BioMart Biomarker provides comprehensive flow cytometry services utilizing our expertise, cutting-edge technology and commitment to quality. Whether it's immunophenotyping, cell counting, or numerous cell function assessments, we offer solutions tailored to meet the diverse needs of researchers. For more service-related information, please feel free to contact us.
Reference:
- Barnard, R.M. Flow cytometry: a flexible tool for biomarker research. Bioanalysis. 2012, 4(20): 2471-83.
