Fas is a transmembrane protein involved in the signal transduction pathway of apoptosis, and the signal transduction pathway mediated by it is essential for the maintenance of immune system homeostasis. Fas is an important biomarker involved in the research of immune-related diseases. Creative BioMart Biomarker provides fast, efficient, and sensitive Fas testing services for customers engaged in disease research and basic research to help advance customers' related research. Our service is not suitable for private medical diagnosis and testing.
Fas and Immune System
Fas, also known as cluster of differentiation 95 (CD95), apoptosis antigen 1 (APO-1), and tumor necrosis factor receptor superfamily member 6 (TNFRSF6), is a transmembrane protein that can trigger apoptosis signal transduction pathways. Fas is a death receptor located on the surface of a variety of cells. It is encoded by the FAS gene and belongs to the tumor necrosis factor (TNF) receptor family. Fas protein contains three domains, which are a cysteine-rich extracellular domain, a transmembrane domain consisting of 17 amino acids, and a cytoplasmic domain consisting of 145 amino acids and containing a death domain (DD). Fas interacts with its ligand FasL (a member of the TNF family) to activate the caspase cascade, thereby inducing the initiation of apoptosis. FasL is a 40kDa protein located on the membrane. FasL can recruit the adaptor protein Fas-associated death domain (FADD), combine with procaspase-8, and form a death-inducing signal complex (DISC). Activated caspase-8 can directly cleave caspase-3 and trigger the initiation of the caspase cascade, which ultimately leads to cell death. Fas-FasL participates in many physiological and pathological processes mediated by programmed cell death, and plays an important role in maintaining immune homeostasis. The Fas-FasL-mediated cell death signal pathway plays an important role in the deletion of autoreactive lymphocytes during the development of the immune system and the deletion of activated lymphocytes during the immune response. Therefore, Fas-mediated apoptosis plays a key role in the immune system, and it also has research value in immune-related diseases, such as multiple sclerosis, systemic lupus erythematosus, and rheumatoid arthritis.
Figure 1. Schematic representation of the Fas–FasL pathway (Volpe, E.; et al. 2016)
Application of Fas Detection
Fas levels can be used as biomarkers in researches related to multiple sclerosis (MS), systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), Sjogren’s syndrome (SS), autoimmune lymphoproliferative syndrome (ALPS), etc.
Our Advantages
- Guarantee high accuracy and sensitivity for Fas detection
- Ensure high repeatability of Fas detection
- Experienced biomarker detection technical team
- Strictly control each specific experimental step to ensure accurate and reliable test results in each sample
- Short turnaround time to accelerate your research
- More favorable prices in the biomarker testing service market can help reduce research costs
Detection Technologies
Samples
- Sample types: serum, plasma, cell culture supernatants, tissue homogenates, etc.
- Target species: human, mouse, rabbit, rat, etc.
Workflow of Biomarker Detection at Creative BioMart Biomarker
At Creative BioMart Biomarker, we offer high-quality Fas detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:
- Yamada, A.; et al. Dual role of Fas/FasL-mediated signal in peripheral immune tolerance. Frontiers in Immunology. 2017, 8: 403.
- Volpe, E.; et al. Fas–Fas ligand: checkpoint of t cell functions in multiple sclerosis. Frontiers in Immunology. 2016, 7: 382.
