DNA methylation analysis is a powerful tool in the field of biomarker analysis, providing valuable insights into various biological processes and disease conditions. Creative BioMart Biomarker provides advantageous DNA methylation analysis services to facilitate biomarker discovery and validation.
DNA Methylation and Biomarkers
DNA methylation is an epigenetic modification that involves the covalent addition of a methyl group to the 5-carbon position of cytosine residues in DNA. DNA methylation can alter genetic expression without changing the DNA sequence. This modification plays a crucial role in regulating gene expression and is associated with various physiological and pathological processes. DNA methylation reactions are divided into de novo methylation and maintenance methylation.
DNA methylation analysis can examine DNA methylation patterns in specific genomic regions or the entire genome, revealing the association between DNA methylation changes and biological phenomena, including disease development, progression, and treatment response. DNA methylation analysis has been widely used in biomarker research in various fields of medicine. By studying DNA methylation patterns, potential biomarkers of diagnostic, prognostic, or predictive value for various diseases can be identified.
Figure 1. The main method for 5-methyl cytosine analysis after treatment with bisulfite. (Khodadadi, E.; et al. 2021)
DNA Methylation Analysis Services Offered by Creative BioMart Biomarker
Creative BioMart Biomarker is at the forefront of providing comprehensive DNA methylation analysis services for biomarker discovery and validation. Our advanced technology and expertise enable accurate and reliable assessment of DNA methylation patterns, facilitating the identification of novel biomarkers for a variety of diseases and conditions. Our services include experimental design, sample preparation, DNA methylation analysis, data analysis and interpretation, biomarker validation.
- Bisulfite Conversion: Bisulfite treatment converts unmethylated cytosines to uracil while leaving methylated cytosines unchanged. Subsequent sequencing or PCR-based methods can detect and quantify the methylation status of specific DNA regions.
- Methylation-specific PCR (MSP): Uses primers specifically designed to amplify methylated or unmethylated DNA regions. By comparing the amplification patterns, the methylation status of target regions can be determined.
- Sequencing Technologies: Whole genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS) are examples of next-generation sequencing (NGS)-based methods that provide a genome-wide view of DNA methylation patterns. These methods enable high-throughput and comprehensive analysis, enabling the identification of novel methylation patterns and the discovery of potential biomarkers with high precision.
- WGBS Service
- RRBS Service
- Methylated DNA Immunoprecipitation Sequencing (MeDIP-Seq) Service
- Oxidative Bisulfite Sequencing (oxBS-seq) Service
The Flow of DNA Methylation Analysis
Our Advantages
- Extensive expertise in DNA methylation analysis ensures the highest quality results and interpretation.
- Perform high-throughput DNA methylation analysis with exceptional accuracy and efficiency, providing robust and comprehensive data for biomarker discovery and validation.
- Customized solutions. Whether it is the selection of sample type, the selection of DNA methylation analysis platform, or the customization of data analysis process, we can provide flexible solutions to meet the specific needs of customers.
DNA methylation analysis advances biomarker research by identifying informative DNA methylation patterns associated with various biological processes and diseases. Creative BioMart Biomarker provides comprehensive DNA methylation analysis services, including experimental design, high-throughput analysis, and data analysis. Please feel free to contact us for more detailed service information.
Reference:
- Khodadadi, E.; et al. Current Advances in DNA Methylation Analysis Methods. Biomed Res Int. 2021, 9: 8827516.
