Cartilage oligomeric matrix protein (COMP) is a large pentameric glycoprotein that interacts with a variety of extracellular matrix proteins in cartilage and other tissues. COMP plays a role in collagen secretion, fibril formation, chondrocyte proliferation and mechanical strength of tendons. COMP is a biomarker of idiopathic pulmonary fibrosis, cartilage degeneration-associated osteoarthritis (OA) and rheumatoid, and is a prognostic marker for joint injury. Creative BioMart Biomarker is capable to provide our customers with superior COMP detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.
Introduction
COMP is an extracellular matrix (ECM) glycoprotein that was originally isolated from cartilage and later found to expresses in a variety of tissues. COMP, also known as TSP5, is a member of the pentameric subgroup of the thrombospondin gene family, a family of secreted matricellular proteins with multiple functions and common structural organization. Like other thrombospondins, COMP is assembled through its N-terminal domain (NTD). COMP is a pentamer, each monomer contains an N-terminal domain, four Type 2 (T2) epidermal growth factor-like repeats, seven Type 3 (T3) repeats and one C-terminal globular domain (CTD). T2 and T3 repeats as well as CTD binding to calcium ions are critical for correcting protein folding. The T3 repeat contains highly conserved aspartate-rich motifs, each motif encasing two calcium ions. CTD is a lectin-like β sandwich domain consisting of 15 β-strands and also containing four calcium binding sites. COMP plays a role in chondrocyte proliferation, Thrombin inhibition and mechanical stress resistance. Chondrocyte proliferation occurs through the interaction of the EGF domain of COMP. COMP is stored in platelet α-particles and is released and synthesized after platelet activation [44]. COMP binds thrombin to slow down blood clotting by inhibiting thrombin-induced platelet aggregation, activation and retraction, and thrombin-mediated fibrinogen cleavage. Tendon studies have shown that COMP plays an important role in mechanical integrity. COMP is more abundant in load-bearing tendons compared to unloaded tendons, suggesting that COMP can enhance the mechanical strength of ECM tissue. COMP is a marker of cartilage turnover and is associated with joint degeneration. High levels of COMP in serum and synovial fluid are associated with early OA and rheumatoid arthritis (RA), degree of cartilage destruction, and disease progression.
Figure 1. The structure of COMP.
Application of COMP
- High levels of COMP in serum and synovial fluid are associated with early OA and rheumatoid arthritis (RA), degree of cartilage destruction, and disease progression.
Our Advantages
- Accept a wide range of sample types
- Ensure high sensitivity for detecting COMP in different samples
- Ensure high accuracy and repeatable COMP detection
- Ensure a wide kinetic range to detect samples of different concentrations
- Short experimental period
Workflow of COMP Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the COMP detection procedure to ensure high sensitivity, high accuracy and repeatable COMP detection.
Please feel free to contact us if you would like to know more about COMP detection. At Creative BioMart Biomarker, we not only provide high-quality COMP detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
Reference:
- Posey, K.L.; et al. Cartilage oligomeric matrix protein: COMPopathies and beyond. Matrix Biology. 2018, 71-72: 161-173.
