Cytokeratin 18 (CK-18) is an intracellular protein related to cell death, which can be used as a potential biomarker in the research of sepsis, autoimmune hepatitis, etc. Creative BioMart Biomarker has a number of advanced technology platforms that can be used for biomarker detection, and can provide high-quality CK-18 detection services for scientists both in academia and industry. We can meet the needs of customers to accelerate research progress through highly efficient and highly standardized testing procedures.
CK-18
CK-18 is the main intermediate filament protein in the cytoskeleton, and is an intracellular protein with high expression in a variety of epithelial cells. CK-18 participates in the cell death pathway and is an important biomarker for evaluating cell death. Most intracellular CK-18 is insoluble. During cell death, it can be released into the blood as the cell ruptures. During cell apoptosis, CK-18 is catalyzed by caspase to undergo hydrolysis. Caspase cleaves CK-18 into three fragments at two sites and releases the resulting fragments into the blood. Therefore, detecting the concentration of the caspase-cleaved fragments of CK18 in the blood can reflect the degree of apoptosis. The M30 assay is based on this principle using a monoclonal antibody that can recognize the neoepitope Asp396 of CK-18. The CK-18 M65 analysis method is used to assess total cell death. The combination of M65 and M30 assays can monitor the proportion of apoptosis in total cell death and evaluate the situation of cell necrosis. Apoptosis increases in patients with sepsis, and this may cause multiple organ failure and even death in patients. Therefore, CK-18 as a biomarker that can be used to monitor apoptosis has also been used in the study of sepsis.
Figure 1. The difference between CK18, CK M30 assay, CK M65 assay, and CK M65ED assay (Qian, et al. 2020)
Application of CK-18 Detection
CK-18 levels can be used as biomarkers in researches related to sepsis, autoimmune hepatitis (AIH), nonalcoholic fatty liver disease (NAFLD), cardiovascular disease, etc.
Our Advantages
- Guarantee high accuracy and sensitivity for CK-18 detection
- Ensure high repeatability of CK-18 detection
- Experienced biomarker detection technical team
- Strictly control each specific experimental step to ensure accurate and reliable test results in each sample
- Short turnaround time to accelerate your research
- More favorable prices in the biomarker testing service market can help reduce research costs
Detection Technologies
Samples
- Sample types: serum, plasma, tissue homogenates, cell culture supernatants, etc.
- Target species: human, mouse, bovine, rabbit, rat, etc.
Workflow of Biomarker Detection at Creative BioMart Biomarker
At Creative BioMart Biomarker, we offer high-quality CK-18 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:
- Qian, L. L.; et al. Elevated Serum Level of Cytokeratin 18 M65ED Is an Independent Indicator of Cardiometabolic Disorders. Journal of Diabetes Research. 2020, 2020: 5198359.
- Leonardo, L.; et al. Serum levels of caspase-cleaved cytokeratin-18 and mortality are associated in severe septic patients: pilot study. Plos One. 2014, 9(10): e109618.
