In the field of biomedical research, understanding the complex mechanisms of cell proliferation is crucial. Cell proliferation assays are powerful tools for studying cell division and replication rates, revealing important biological processes. Creative BioMart Biomarker facilitates biomarker-related research by providing comprehensive cell proliferation assay services.
Cell proliferation is the basis of organism growth, development, reproduction and heredity, and is the most basic physiological activity of cells. Cell proliferation assays include a range of laboratory techniques used to measure cell division and growth rates. These assays provide valuable insights into a variety of biological phenomena, including tissue development, wound healing, and cancer progression. By quantifying the extent of cell proliferation, researchers can decipher key cellular behaviors and uncover underlying mechanisms that drive disease progression. Cell proliferation assays can be used to identify potential cancer biomarkers, evaluate potential therapeutic agents, and for regenerative medicine research.
Creative BioMart Biomarker offers a comprehensive range of cell proliferation assays to meet the specific needs of biomarker research. Our assays incorporate a variety of methods including MTT assays, BrdU incorporation assays, cell counts, and EdU click chemistry assays.
The MTT assay is based on the reduction of a yellow tetrazolium dye (MTT) by metabolically active cells to form a purple formazan product. The intensity of the color produced is directly proportional to the number of viable cells in the culture.
Bromodeoxyuridine (BrdU) is a thymidine analog that is incorporated into the DNA of dividing cells during the S phase of the cell cycle. Proliferating cells can be identified and quantified using specific antibodies to detect BrdU incorporation.
This method involves counting the total number of cells in the population at different time points. It can be performed manually using a hemocytometer or automatically using cell counting instruments. The change in cell number over time indicates the rate of cell proliferation.
EdU (5-ethynyl-2'-deoxyuridine) is another thymidine analog that is incorporated into newly synthesized DNA during cell division. EdU is then labeled using click chemistry, allowing visualization and quantification of proliferating cells.
By partnering with Creative BioMart Biomarker, you can leverage comprehensive services and custom solutions to unravel the complexities of cell proliferation and advance biomarker research. Please feel free to contact us for more service information.
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