Carbonic anhydrase IX (CA9) is a transmembrane protein involved in cancer progression and can indicate hypoxia. In addition to being associated with renal cell carcinoma (RCC), it is also abnormally expressed in common tumors such as non-small cell lung cancer (NSCLC) and breast cancer. Creative BioMart Biomarker has been committed to providing customers with high-quality cancer biomarker test services, including CA9 detection services. Our technical team has rich experience and can offer customers test results with high accuracy, sensitivity and repeatability. Fast testing and reliable results can effectively help customers shorten the research cycle.
CA9 is a member of the α carbonic anhydrase family that belongs to zinc metalloenzymes. CA9 is closely related to cancer, acidic pKa optimum, etc., and is involved in adjusting pH and maintaining acid-base balance in the body. CA9 can catalyze the reversible conversion of CO2 to bicarbonate ions and protons through its extracellular enzyme domain. The bicarbonate ion is uploaded by the adjacent bicarbonate transporter and transported into the cytoplasm. The bicarbonate can react with intracellular protons to generate CO2, which leaves the cell by diffusion. This process helps to adjust the pH to a value suitable for signal transduction and proliferation. The protons produced by CA9 catalyzing CO2 contribute to extracellular acidification. At the same time, CA9 can promote the output of lactic acid through a non-catalytic mechanism to further promote the acidification of the pericellular milieu, which helps cancer cells invade surrounding tissues. In addition, the transcriptional regulation of CA9 gene is regulated by hypoxia-inducible factor 1 (HIF-1), and the oncogenic signal affects the expression and activation of HIF, resulting in CA9 gene transcription will also increase with the activation of related signaling pathways and tyrosine kinase. Tissue hypoxia is mediated by the HIF signaling cascade, and hypoxia is associated with tumor aggressiveness, poor prognosis, and resistance to conventional therapy. In general, CA9 is a hypoxia and cancer biomarker with research potential.
Figure 1. Schematic model of the CA IX role in pH regulation in hypoxic cancer cells (Pastorekova, et al. 2019)
Plasma and serum CA9 levels can be used as biomarkers in researches related to breast cancer, RCC, NSCLC, esophageal cancer, cervical cancer, etc.
Creative BioMart Biomarker strictly controls each specific experimental step in the detection procedure to ensure accurately quantify the level of CA9 in each sample.
At Creative BioMart Biomarker, we offer high-quality CA9 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.