C4 Detection Service

7α-Hydroxy-4-cholesten-3-one (C4) is a key intermediate in the major pathway of bile acid biosynthesis from cholesterol. It can be used as a biomarker to monitor bile acid malabsorption and can be detected in serum and plasma. Creative BioMart Biomarker is capable to provide our customers with superior C4 detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.


Bile acids are synthesized by cholesterol through the liver, and the synthesis process is complicated, including at least 14 enzymatic reactions. In humans, any failure of these enzymatic reactions leads to the accumulation of intermediates in the bile acid synthesis pathway, as well as the absence of normal primary bile acids, cholic acids (CA) and chenodeoxycholic acid (CDCA). Biosynthesis of cholesterol into primary bile acids requires sequential 7α-hydroxylation catalyzed by cholesterol 7α-hydroxylase (Cyp7A1), and then C27 3β-hydroxy- Δ5-C27-steroid oxidoreductase (HSD3B7) catalyzes C-3 oxidation and double bond migration to form C4, which is CDCA precursor. C4 can be metabolized to 7α, 12α-dihydroxy-4-cholesten-3-one by microsomal steroid 12α-hydroxylase Cyp8b1 and then metabolized to CA. Alternatively it can be converted to 5β-cholestane-3α, 7α-diol and then converted to CDCA. The level of serum C4 reflects the activity of the bile acid synthesis pathway, and its level changes during the day because bile acid synthesis has a circadian rhythm. C4 is a key intermediate in bile acid biosynthesis, and studies have shown that plasma C4 levels are highly correlated with the rate of bile acid biosynthesis and are therefore biomarkers for detecting increased bile acid biosynthesis. This determination is useful for patients with idiopathic diarrhea to determine if bile acid malabsorption is present.

Application of C4

Serum, plasma levels of C4 can be used as a biomarker for the diagnosis of bile acid malabsorption in pediatric inflammatory bowel disease, idiopathic diarrhea and so on.

Our Advantages

  • Accept a wide range of sample types
  • Provide multiple detection methods, including ELISA, HPLC, LC-MS/MS
  • Ensure high sensitivity for detecting C4 in different samples
  • Ensure high accuracy and repeatable C4 detection
  • Ensure a wide kinetic range to detect samples of different concentrations
  • Short experimental period

Workflow of C4 Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the C4 detection procedure to ensure high sensitivity, high accuracy and repeatable C4 detection.


Please feel free to contact us if you would like to know more about C4 detection. At Creative BioMart Biomarker, we not only provide high-quality C4 detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.


  1. Ogawa, S.; et al. An efficient synthesis of 7a,12a-dihydroxy-4-cholesten-3-one and its biological precursor 7a-hydroxy-4-cholesten-3-one: Key intermediates in bile acid biosynthesis. Steroids.2013, 78(9): 927-37.


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