C2C Detection Service

Type II collagen is the major structural protein of cartilage, accounting for about 50% of the extracellular matrix. Studies have shown that fragments derived from collagen degradation are potential markers for remodeling cartilage pathologies. Among various type II collagen degradation products, a neoepitope (Collagen Type II Cleavage, C2C) produced by intra-helix cleavage of collagenase has been well studied in vivo. Creative BioMart Biomarker is capable to provide our customers with superior C2C detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.


The articular cartilage matrix is mainly composed of type II collagen and proteoglycans such as aggrecan. Collagen fibrils provide tensile strength to maintain tissue integrity, while aggrecan interweaves with collagen fibrils to increase compression stiffness. Type II collagen is important for endochondral bone formation, growth, and normal joint function. Over-cleavage of type II collagen by collagenase is a critical step leading to destruction of the cartilage matrix. Articular cartilage matrix destruction is a feature of osteoarthritis (OA) that may be associated with unbearable pain and daily life difficulties such as walking, up and down stairs, and other movements that accompany knee flexion or stretching. Once a radiographic change occurs, the development of cartilage damage cannot be prevented. Therefore, in order to prevent the development of OA, early cartilage damage must be detected. Collagenase-cleaved type II collagen neoepitepe (C2C) is a biomarker that responds to type II collagen lysis in patients with anterior cruciate ligament (ACL) injury or OA. Neoepitope C2C located at the C-terminus of the 3/4 length fragment, is 10 amino acids long [GGEGPP(OH)GPQG]. This neoepitope originally designated COL2-3/4Clong.

Amino acid sequence of C2C.Figure 1. Amino acid sequence of C2C.

Application of C2C

  • Serum and urine C2C levels is biomarkers for the diagnosis of OA and ACL.

Our Advantages

  • Accept a wide range of sample types
  • Ensure high sensitivity for detecting C2C in different samples
  • Ensure high accuracy and repeatable C2C detection
  • Ensure a wide kinetic range to detect samples of different concentrations
  • Short experimental period

Workflow of C2C Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the C2C detection procedure to ensure high sensitivity, high accuracy and repeatable C2C detection.

C2C Detection Service

Please feel free to contact us if you would like to know more about C2C detection. At Creative BioMart Biomarker, we not only provide high-quality C2C detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.


  1. Sobue, Y.; et al. Prediction of progression of damage to articular cartilage 2 years after anterior cruciate ligament reconstruction: use of aggrecan and type II collagen biomarkers in a retrospective observational study. Arthritis Research & Therapy. 2017, 19: 265.
  2. Vilar, J.M.; et al. Serum collagen type II cleavage epitope and serum hyaluronic acid as biomarkers for treatment monitoring of dogs with hip osteoarthritis. PLoS ONE. 2016, 11(2): e0149472.
  3. Billinghurst, R.C.; et al. Immunoassay for collagenase-mediated cleavage of types I and II collagens. Methods in Molecular Biology. 2001, 151: 457-472.


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