C1C2, also known as Col2-3/4Cshort, is 7 amino acids long [GPP(OH)GPQG], which originates from the cleavage of type I collagen and type II collagen. C1C2 is considered to be an OA diagnostic marker, and serum C1C2 levels are elevated in OA patients. Creative BioMart Biomarker is capable to provide our customers with superior C1C2 detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.
Introduction
Type II collagen is a homotrimer of the a1 (II) chain that undergoes hydroxylation, interchain disulfide bonds and triple helix. This molecule is then secreted into the extracellular matrix of the cartilage. With the development of osteoarthritis (OA), the joint components including matrix and cartilage are degraded by proteases, and collagenase cleaves type II collagen, producing two fragments: a 3/4 length fragment and a 1/4 length fragment. Neoepitope C1C2 is located at the C-terminus of the longer fragment. C1C2 is a carboxy-terminal cleavage neoepitope on the collagen 3/4 fragment. The name "C1C2" indicates that the neoepitope is not specific for type II collagen and is also produced by collagenase cleavage of type I collagen, exposing five amino terminal residues on the 3/4 fragment. C1C2 is a secondary cleavage product of C2C. This neoepitope is also known as COL2-3/4short and represents the length of the epitope, not the length of the fragment in which the epitope is located. As a diagnostic marker, the level of C1C2 is elevated in the cartilage of patients with osteoarthritis (OA). In a cartilage explant system, collagenase cleavage of type II collagen in OA cartilage samples was increased, resulting in higher levels of C1C2, and this increase was blocked by collagenase inhibitors. Also, studies have shown that serum C1C2 level was associated with incident radiographic knee OA.
Figure 1. Amino acid sequence of C1C2.
Application of C1C2
- Serum, urine levels of C1C2 can be used as biomarkers for the diagnosis of OA.
Our Advantages
- Accept a wide range of sample types
- Provide multiple detection methods
- Ensure high sensitivity for detecting C1C2 in different samples
- Ensure high accuracy and repeatable C1C2 detection
- Ensure a wide kinetic range to detect samples of different concentrations
- Short experimental period
Workflow of C1C2 Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the C1C2 detection procedure to ensure high sensitivity, high accuracy and repeatable C1C2 detection.
Please feel free to contact us if you would like to know more about C1C2 detection. At Creative BioMart Biomarker, we not only provide high-quality C1C2 detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
References:
- Lennerová, T.; et al. Biomarkers of hand osteoarthritis. Rheumatol Int. 2017, doi: 10.1007/s00296-017-3864-5.
- Birmingham, J.D.; et al. Collagen biomarkers for arthritis applications. Biomarker Insights. 2006, I: 61–76.
- Billinghurst, R.C.; et al. Immunoassay for collagenase-mediated cleavage of types I and II collagens. Methods in Molecular Biology. 2001, 151: 457-472.
