Bone morphogenetic protein 9 (BMP-9) is a growth factor associated with osteogenesis, fibrosis, angiogenesis, and lipopolysaccharide (LPS)-induced inflammation, which is abnormally expressed in the inflammatory environment. Therefore, BMP-9 has the potential to become a biomarker for inflammation. As a technical service company in the field of biomarkers, Creative BioMart Biomarker has many years of experience in biomarker testing and can provide customers with accurate and reliable BMP-9 detection services.
Introduction
BMP-9 is an effective growth factor, which is also known as growth differentiation factor 2 (GDF2). The BMP family includes multiple subtypes (BMP-2, BMP-4, BMP-6, BMP-7, BMP-9, BMP-10, BMP-15 etc.), in which abnormalities of BMP-2, BMP-4, BMP-6, and BMP-9 signaling are associated with inflammation. BMP-9 is mainly produced by the liver and is associated with multiple physiological activities such as iron homeostasis, hepatocyte growth, glucose and fatty acid metabolism, bone formation, cholinergic neuronal differentiation, angiogenesis, tumor progression, etc. BMP-9 belongs to the transforming growth factor beta (TGF-β) superfamily. The TGF-β family is composed of multiple functional proteins that can activate downstream signal transduction cascades such as the ALK-Smad pathway. The activation of these pathways (e.g. ALK5–Smad2/3 and ALK1–Smad1/5/8 pathways) may lead to the inhibition or activation of endothelial cell migration and proliferation. BMP-9 can bind with high affinity to its specific type I receptor, activin receptor-like kinase 1 (ALK1), and induce the phosphorylation of Smad1/5, at the same time, inhibit the proliferation and migration of endothelial cells induced by basic fibroblast growth factor (FGF) and angiogenesis induced by vascular endothelial growth factor (VEGF). BMP-9 is associated with vascular permeability and angiogenesis, while chronic systemic inflammation is associated with many cardiovascular diseases. In the LPS-induced inflammatory environment, the expression level of BMP-9 was significantly down-regulated, and the restoration of its expression level stimulated ALK-1-mediated signaling pathways, which ultimately led to anti-inflammatory and endothelial protection.
Figure 1. BMP-9/Smad signaling (Mostafa, et al. 2019)
Application of BMP-9 Detection
Plasma and serum BMP-9 levels can be studied as a biomarker in researches related to acute respiratory distress syndrome, atherosclerosis, LPS-induced tissue injury, etc.
Our Advantages
- Guarantee high accuracy and sensitivity for BMP-9 detection
- Ensure high repeatability of BMP-9 detection
- Short turn-around time of detection service
- Competitive price in the market of BMP-9 detection services
- Multiple technology platforms for BMP-9 test services (ELISA, CLIA)
- Accept a wide range of sample types (plasma, serum, cell culture supernatants, etc.)
Workflow of Biomarker Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the detection procedure to ensure accurately quantify the level of BMP-9 in each sample.
At Creative BioMart Biomarker, we offer high-quality BMP-9 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:
- Suzuki, Y.; et al. BMP-9 induces proliferation of multiple types of endothelial cells in vitro and in vivo. Journal of Cell Science. 2010, 123(10): 1684-92.
- Mostafa, S.; et al. The wonders of BMP9: from mesenchymal stem cell differentiation, angiogenesis, neurogenesis, tumorigenesis, and metabolism to regenerative medicine. Genes & Diseases. 2019, 6(3): 201-223.
- Li, W.; et al. Circulating BMP9 protects the pulmonary endothelium during inflammation-induced lung injury in mice. bioRxiv. 2020, doi: 10.1101/2020.05.12.088880.
