B-cell activating factor (BAFF) is a protein involved in immune regulation and abnormally expressed in some immune diseases. Therefore, it may be used as a potential biomarker in the research of immune diseases. Creative BioMart Biomarker has a wealth of biomarker detection experience, which can provide researchers with accurate, fast and reliable detection results and help customers accelerate research progress.
BAFF
BAFF, also known as B Lymphocyte Stimulator (BLyS), is a member of tumor necrosis factor ligand superfamily. BAFF is produced by monocytes, macrophages, neutrophils and activated T lymphocytes. BAFF can regulate the immune response and has the biological function of promoting the survival and maturation of B cells. In addition, BAFF can also promote the proliferation, differentiation and activation of T cells. BAFF can be expressed in two forms, one is membrane-bound protein (mBAFF) and the other is soluble protein (sBAFF). sBAFF is released after the proteolytic cleavage of furin protease site by metalloproteases. It can exist in the form of trimers or polymers, as well as glycosylated or non-glycosylated forms. BAFF can bind to three receptors, including BAFF receptor (BAFF-R), B cell maturation antigen (BCMA), transmembrane activator and calcium modulator and cytophilin ligand interactor (TACI). BAFF-R, TACI and BCMA are expressed in immature B cells, mature memory B cells and plasma cells, respectively. The combination of BAFF and BAFF-R triggers the non-canonical nuclear factor-κB (NF-κB) signaling pathway, and the BAFF-R signaling pathway is essential for peripheral B cell survival and differentiation, germinal centre formation, plasma cell survival and immunoglobulin class switching. At the same time, the balance between the signal pathway mediated by BCMA and BAFF-R is related to the development of Tfh cells, so BAFF may regulate autoimmunity through the expansion of Tfh cells. In addition, BAFF has elevated expression in both SLE and AAV patients and has been used as a target for research on these diseases.
Figure 1. BAFFR, TACI and BCMA in B cell development (Smulski, et al. 2018)
Application of BAFF Detection
BAFF levels can be used as biomarkers in researches related to systemic lupus erythematosus (SLE), Sjogren’s syndrome (SS) and antineutrophil cytoplasmic antibody-associated vasculitis (AAV), etc.
Our Advantages
- Guarantee high accuracy and sensitivity for BAFF detection
- Ensure high repeatability of BAFF detection
- Experienced biomarker detection technical team
- Strictly control each specific experimental step to ensure accurate and reliable test results in each sample
- Short turnaround time to accelerate your research
- More favorable prices in the biomarker testing service market can help reduce research costs
Detection Technologies
Samples
- Sample types: serum, plasma, cell culture supernatants, etc.
- Target species: human, mouse, bovine, canine, rat, etc.
Workflow of Biomarker Detection at Creative BioMart Biomarker
At Creative BioMart Biomarker, we offer high-quality BAFF detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:
- Thompson, N.; et al. Exploring BAFF: its expression, receptors and contribution to the immunopathogenesis of Sjögren’s syndrome. Rheumatology. 2016, 55(9): 1548–1555.
- Nakayamada, S.; Tanaka, Y. BAFF- and APRIL-targeted therapy in systemic autoimmune diseases. Inflammation & Regeneration. 2016, 36: 6.
- Smulski, C. R.; Eibel, H. BAFF and BAFF-receptor in B cell selection and survival. Frontiers in Immunology. 2018, 9: 2285.
