7-Ketocholesterol is the most important oxidation product of cholesterol. 7-ketocholesterol is as an endogenous regulator of the rate-limiting enzyme in cholesterol biosynthesis and bile acid synthesis. Excessive 7-ketocholesterol may cause lipid metabolism disorder, atherosclerosis and even carcinogenesis, so it is often used as a marker for cholesterol oxidation. Creative BioMart Biomarker is capable to provide our customers with superior 7-ketocholesterol detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.
7-Ketocholesterol is produced from cholesterol via the epimeric cholesterol 7-hydroperoxides (cholest-5-ene-3β-ol-7-hydroperoxide), which decomposes into the epimeric 7-hydroxycholesterols (cholest-5-ene-3β, 7-diol) and 7-ketocholesterol. Cholesterol is a monounsaturated component of the cell membrane that is involved in the permeability and fluidity. Due to the presence of a double bond (carbon 5) in cholesterol, various cholesterol oxidation products can be produced by different reaction mechanisms and pathways. Excessive intake of cholesterol oxidation products may be harmful to health because cholesterol oxidation products may have negative effects on the body, such as atherosclerosis, cytotoxicity, mutation and carcinogenesis, and may be involved in some chronic and degenerative diseases, as well as disorders of cellular function and lipid metabolism. 7-Ketocholesterol is the major oxidation product of cholesterol found in human atherosclerotic plaques and is more likely to cause atherosclerosis than cholesterol in some animal studies. 7-Ketocholesterol inhibits the rate-limiting enzyme cholesterol 7α-hydroxylase in bile acid biosynthesis and strongly inhibits the rate-limiting enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) in cholesterol biosynthesis. Because it is easy to form and is the most representative hydroxysterol, 7-ketocholesterol is often used as a marker for cholesterol oxidation.
Plasma level of 7-ketocholesterol can be used as a biomarker to monitor cholesterol oxidation and predict the risk of atherosclerosis and cardiovascular events.
Creative BioMart Biomarker strictly controls each specific experimental step in the 7-ketocholesterol detection procedure to ensure high sensitivity, high accuracy and repeatable 7-ketocholesterol detection.
Please feel free to contact us if you would like to know more about 7-ketocholesterol detection. At Creative BioMart Biomarker, we not only provide high-quality 7-ketocholesterol detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.