Creative BioMart Biomarker has established itself as a preeminent purveyor of biomarker services, specifically through the employment of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE). This powerful proteomics technique allows for the accurate and reproducible comparison of protein expression levels between samples on a single gel.
2D-DIGE and Biomarker
Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) is a proteomics technique that combines the high-resolution separation of proteins provided by two-dimensional gel electrophoresis (2D-GE) with the precision of fluorescence labeling. By labeling different samples with distinct fluorescent dyes (e.g., Cy3 and Cy5), 2D-DIGE enables samples to be run together on the same gel and compared directly.
In biomarker studies, 2D-DIGE has been widely utilized, including in cancer research, drug discovery, and disease monitoring. 2D-DIGE has been applied to identify differentially expressed proteins between cancer and normal tissue samples, providing a potential means for the identification of biomarkers for diagnosis, prognosis, and treatment. In drug discovery, 2D-DIGE has been employed to identify target proteins affected by a drug candidate, providing invaluable insight into its mode of action and potential side effects. 2D-DIGE has also been employed to identify changes in protein expression between different stages of a disease.
Figure 1. The 2D-DIGE concept. (Diez, R.; et al. 2010)
2D-DIGE Services
Creative BioMart Biomarker's experience and knowledge in the field of biomarkers allows us to provide expert guidance for 2D-DIGE analysis. We provide one-stop services for protocol design, sample processing and data analysis, and ensure accurate, reproducible and reliable test results. We also provide 2D-DIGE-related services such as 2D-DIGE-MS to meet the research needs of different customers.
We implement strict quality control procedures in our projects and provide detailed results reports to give you a complete understanding of our procedures and the results we obtained.
Our Advantages
- Our team of scientists has extensive experience in biomarker discovery and validation and has been involved in various projects.
- We provide one-stop services including study design, sample selection and data analysis.
- Using the latest technology and equipment ensures accurate and reliable results.
- A range of services that can be customized to meet your specific needs, ensuring you have the exact information you need for your biomarker research.
- Competitive pricing and fast turnaround time.
At Creative BioMart Biomarker, we are dedicated to helping you achieve your research goals, and our 2D-DIGE services are just one of the many ways we do this. Contact us today to learn more about how we can help you take your research to the next level.
Reference:
- Diez, R.; et al. 2-D Fluorescence Difference Gel Electrophoresis (DIGE) in Neuroproteomics. In: Alzate O, editor. Neuroproteomics. Boca Raton (FL): CRC Press/Taylor & Francis; 2010. Chapter 4.
