25-Hydroxy Vitamin D Detection Service

Vitamin D deficiency is a common symptom in numerous people in the world, affecting the health of many people, so the detection of vitamin D is becoming more and more important. 25-hydroxy vitamin D is a metabolite of vitamin D and is considered to be the best indicator of vitamin D. Creative BioMart Biomarker is capable to provide our customers with superior 25-hydroxy vitamin D detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.


The main function of vitamin D is to maintain calcium balance in the body by increasing intestinal absorption of calcium. Calcium absorbed from the intestine and reabsorbed from the kidney promotes bone mineralization. In addition to its role in calcium and phosphate metabolism, Vitamin D also plays an important role in vascular, inflammation, tumors, and neurodegenerative diseases. Vitamin D deficiency is associated with a variety of diseases, including osteoporosis, rickets, cardiovascular diseases, autoimmune diseases, multiple sclerosis and cancer, so the need to assess the status of vitamin D has increased dramatically.

25-Hydroxy Vitamin D Detection ServiceFigure. 1 The 25-OHD signaling pathway.

The vitamin D status in the clinical environment is currently assessed primarily by measuring the total amount of 25-hydroxy vitamin D (25-OHD), which reflects the total serum vitamin D in three forms: 1) binding to vitamin D binding protein (DBP), accounting for 85% of the total; 2) binding to albumin, accounting for the total 15%; 3) unbound protein, accounting for 0.03% of the total. 25-OHD is the most abundant vitamin D metabolite and is considered to be the best indicator of vitamin D. Serum 25-OHD is associated with clinical outcomes such as bone mineralization, fracture risk, falls risk, all-cause mortality, and cardiovascular disease. Serum 25-OHD levels <50 nmol/L (20 ng/L) for vitamin D deficiency, serum 25OHD levels >75 nmol/L (30 ng/L) are normal, 50-75 nmol/L (20-30 ng/L) is vitamin D insufficient. Recent studies have identified new markers of vitamin D, including vitamin D metabolite ratio (VMR) (ratio between 25-OHD and 24,25-dihydroxy vitamin D), bioavailable 25-OHD (not Binding to vitamin D binding protein) and free 25-OHD (neither bound to DBP nor bound to ALB).

Application of 25-Hydroxy Vitamin D

  • Serum 25-OHD level as a biomarker to predict vitamin D status. 25-OHD may also be the preferred marker of the ability of tissues to produce complete 1,25-OH2D autocrine/paracrine vitamin D signals.

Our Advantages

  • Provide multiple 25-OHD detection methods, including ELISA, RIA, HPLC
  • Ensure high sensitivity for detecting 25-OHD in different samples
  • Ensure high accuracy and repeatable 25-OHD detection
  • Ensure a wide kinetic range to detect samples of different concentrations
  • Short experimental period

Workflow of 25-Hydroxy Vitamin D Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the 25-OHD detection procedure to ensure high sensitivity, high accuracy and repeatable 25-OHD detection.

25-Hydroxy Vitamin D Detection Service

Please feel free to contact us if you would like to know more about 25-OHD detection. At Creative BioMart Biomarker, we not only provide high-quality 25-OHD detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.


  1. Goltzman D. Functions of vitamin D in bone. Histochemistry and Cell Biology. 2018, doi:10.1007/s00418-018-1648-y.
  2. Herrmann M.; et al. Assessment of vitamin D status – a changing andscape. Clin Chem Lab Med. 2017, 55(1): 3-26
  3. Lai J.C.; et al. Total 25(OH) vitamin D, free 25(OH) vitamin D and markers of bone turnover in cirrhotics with and without synthetic dysfunction. Liver Int. 2015, 35(10): 2294-2300.
  4. Kamineni V.; et al. Association between serum 25-hydroxyvitamin D levels and bone mineral density in normal postmenopausal women. Journal of Midlife Health. 2016, 7:163-8.


Contact Us


Enter your email here to subscribe