1,25-Dihydroxy Vitamin D Detection Service
1,25-Dihydroxy vitamin D is a metabolically active molecule responsible for most of the effects of vitamin D. Serum 1,25-dihydroxy vitamin D is associated with higher bone turnover and poorer bone healthdespite being positively related to 25OHD. Creative BioMart Biomarker is capable to provide our customers with superior 1,25-dihydroxy vitamin D detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.
Introduction
1,25-Dihydroxy vitamin D is derived from 1-hydroxylation of 25-hydroxyvitamin D (25OHD). Endogenous or exogenous vitamin D, circulates in the blood and binds to vitamin D binding protein (DBP), then is converted to 25OHD by a cytochrome P450 mixed-function oxidase (CYP2R1) with 25 hydroxylase activity in the liver. 25OHD binds to DBP, is transported to the kidney, and then 1-hydroxylated by mitochondrial CYP27B1 with 1-hydroxylase activity to produce 1,25-dihydroxy vitamin D.
Figure 1. The 1,25-Dihydroxy vitamin D signaling pathway.
The main role of 1,25-Dihydroxy vitamin D is to increase calcium absorption from the gut, it binds to the vitamin D in the epithelial cells of the duodenum, leads to the synthesis of calcium binding proteins that regulate intestinal calcium absorption. In addition, studies have shown that 1,25-dihydroxy vitamin D may regulate bone calcium absorption through direct action on bone cells. The normal level of 1,25-dihydroxyvitamin D in serum reduces the ratio of RANKL/OPG through VDR in mature osteoblasts, thereby reducing osteoclastic bone resorption and increasing bone formation rate (BFR), resulting in cortical bone and trabecular bone increase. High levels of 1,25-dihydroxy vitamin D act through VDP in less mature osteoblasts, may increase RANKL/OPG, stimulat bone resorption by osteoclasts, and less trabecular bone. High levels of 1,25-dihydroxy vitamin D increase local and systemic inhibitors of bone mineralization in mature osteoblasts and bone cells, can reduce bone mineralization leading to osteomalacia.
Application of 1,25-Dihydroxy Vitamin D
- Serum 1,25-dihydroxy vitamin D level as a biomarker to predict bone formation, bone healthdespite and hypophospatemic rickets. High levels of 1,25-dihydroxy vitamin D can cause osteomalacia.
Our Advantages
- Provide multiple 1,25-dihydroxy vitamin D detection methods, including ELISA, RIA
- Ensure high sensitivity for detecting 1,25-dihydroxy vitamin D
- Ensure high accuracy and repeatable 1,25-dihydroxy vitamin D detection
- Ensure a wide kinetic range to detect samples of different concentrations
- Short experimental period
Workflow of 1, 25-Dihydroxy Vitamin D Detection at Creative BioMart Biomarker
Creative BioMart Biomarker strictly controls each specific experimental step in the 1,25-dihydroxy vitamin D detection procedure to ensure high sensitivity, high accuracy and repeatable 1,25-dihydroxy vitamin D detection.
Please feel free to contact us if you would like to know more about 1,25-dihydroxy vitamin D detection. At Creative BioMart Biomarker, we not only provide high-quality 1,25-dihydroxy vitamin D detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
References:
- Vanderschueren,D.; et al. Active vitamin D (1,25-Dihydroxyvitamin D) and bone health in middle-aged and elderly men: the european male aging study (EMAS). J Clin Endocrinol Metab. 2013, 98(3).
- Goltzman D. Functions of vitamin D in bone. Histochemistry and Cell Biology. 2018, doi:10.1007/s00418-018-1648-y.
