1,25-Dihydroxy vitamin D is a metabolically active molecule responsible for most of the effects of vitamin D. Serum 1,25-dihydroxy vitamin D is associated with higher bone turnover and poorer bone healthdespite being positively related to 25OHD. Creative BioMart Biomarker is capable to provide our customers with superior 1,25-dihydroxy vitamin D detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.
1,25-Dihydroxy vitamin D is derived from 1-hydroxylation of 25-hydroxyvitamin D (25OHD). Endogenous or exogenous vitamin D, circulates in the blood and binds to vitamin D binding protein (DBP), then is converted to 25OHD by a cytochrome P450 mixed-function oxidase (CYP2R1) with 25 hydroxylase activity in the liver. 25OHD binds to DBP, is transported to the kidney, and then 1-hydroxylated by mitochondrial CYP27B1 with 1-hydroxylase activity to produce 1,25-dihydroxy vitamin D.
Figure 1. The 1,25-Dihydroxy vitamin D signaling pathway.
The main role of 1,25-Dihydroxy vitamin D is to increase calcium absorption from the gut, it binds to the vitamin D in the epithelial cells of the duodenum, leads to the synthesis of calcium binding proteins that regulate intestinal calcium absorption. In addition, studies have shown that 1,25-dihydroxy vitamin D may regulate bone calcium absorption through direct action on bone cells. The normal level of 1,25-dihydroxyvitamin D in serum reduces the ratio of RANKL/OPG through VDR in mature osteoblasts, thereby reducing osteoclastic bone resorption and increasing bone formation rate (BFR), resulting in cortical bone and trabecular bone increase. High levels of 1,25-dihydroxy vitamin D act through VDP in less mature osteoblasts, may increase RANKL/OPG, stimulat bone resorption by osteoclasts, and less trabecular bone. High levels of 1,25-dihydroxy vitamin D increase local and systemic inhibitors of bone mineralization in mature osteoblasts and bone cells, can reduce bone mineralization leading to osteomalacia.
Creative BioMart Biomarker strictly controls each specific experimental step in the 1,25-dihydroxy vitamin D detection procedure to ensure high sensitivity, high accuracy and repeatable 1,25-dihydroxy vitamin D detection.
Please feel free to contact us if you would like to know more about 1,25-dihydroxy vitamin D detection. At Creative BioMart Biomarker, we not only provide high-quality 1,25-dihydroxy vitamin D detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.