| Catalog#: |
MK0741R |
| Product Name: |
Recombinant Rat TNF Protein |
| Product Overview: |
Recombinant mature form of rat TNFα (P16599) (Leu 80-Leu 235) was expressed and purified, with an initial Met at the N-terminus. |
| Source: |
E. coli |
| Species: |
Rat |
| Tag: |
N/A |
| Formulation: |
Lyophilized from a sterile solution of PBS, pH 7.4, 5%~8% trehalose and mannitol. |
| Purity: |
>93% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The recombinant rat TNFα consisting of 157 amino acids and has a calculated molecular mass of 17.4 kDa. It migrates as an approximately 15 kDa band in SDS-PAGE under reducing conditions. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70℃. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
