| Catalog#: |
MK0681M |
| Product Name: |
Recombinant Mouse S100a8, His tagged |
| Product Overview: |
Recombinant Mouse S100a8 (P27005) (Pro2-Glu89), fused with a polyhistidine tag at the N-terminus, was produced in E. coli. |
| Description: |
S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular |
| Source: |
E. coli |
| Species: |
Mouse |
| Tag: |
His |
| Formulation: |
Lyophilized from sterile 50mM Tris, 100mM NaCl, 20% Glycerol, pH 8.0.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The recombinant mouse S100a8 consists of 104 amino acids and predicts a molecular mass of 12.3 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70℃. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
