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Recombinant Mouse Egf protein, Fc-tagged

Product Information
Catalog#:  MK0246M
Product Name:  Recombinant Mouse Egf protein, Fc-tagged
Product Overview:  Recombinant Mouse Egf (Asn977-Arg1029) protein was fused to human IgG1 Fc tag at N-terminus and expressed in human 293 cells (HEK293 cells).
Description:  Mouse Egf is the founding member of the EGF-family of growth factors. All the members of this protein family have highly similar structural and functional characteristics. Egf is initially synthesized as a 130 kDa single-pass transmembrane protein containing 9 EGF-like units and 9 LDL-receptor class B repeats. The mature soluble Egf is a 6 kDa peptide corresponding to the Egf unit located proximal to the transmembrane domain.
Source:  HEK293 cells
Species:  Mouse
Tag:  Fc
Formulation:  Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Protein length:  Asn977-Arg1029
Purity:  >95% as determined by SDS-PAGE.
Storage:  Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Reconstitution:  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents.
Molecular Mass:  The protein has a calculated MW of 32.5 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin:  Less than 1.0 EU per μg as determined by the LAL method.
Notes:  Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles.
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