| Catalog#: |
MK0246M |
| Product Name: |
Recombinant Mouse Egf protein, Fc-tagged |
| Product Overview: |
Recombinant Mouse Egf (Asn977-Arg1029) protein was fused to human IgG1 Fc tag at N-terminus and expressed in human 293 cells (HEK293 cells). |
| Description: |
Mouse Egf is the founding member of the EGF-family of growth factors. All the members of this protein family have highly similar structural and functional characteristics. Egf is initially synthesized as a 130 kDa single-pass transmembrane protein containing 9 EGF-like units and 9 LDL-receptor class B repeats. The mature soluble Egf is a 6 kDa peptide corresponding to the Egf unit located proximal to the transmembrane domain. |
| Source: |
HEK293 cells |
| Species: |
Mouse |
| Tag: |
Fc |
| Formulation: |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
| Protein length: |
Asn977-Arg1029 |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The protein has a calculated MW of 32.5 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
