| Product Information | |
|---|---|
| Catalog#: | MK0996M |
| Product Name: | Recombinant Mouse CD73/NT5E Protein, His-tagged |
| Description: | CD73, an ecto-5'-nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. |
| Source: | HEK293 cells |
| Species: | Mouse |
| Tag: | His |
| Formulation: | Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). |
| Protein length: | Trp29-Lys549 |
| Purity: | > 95% as determined by BisTris PAGE. |
| Storage: | Store under sterile conditions at -20 °C to -80 °C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water. |
| Molecular Mass: | 58.6 kDa |
| Stability: | Upon receiving, the product remains stable for 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. |
| Endotoxin: | Less than 1 EU per μg by the LAL method. |
| Notes: | Research Use Only. Not for use in clinical procedures. |
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