| Catalog#: |
MK0756H |
| Product Name: |
Recombinant Human TOP1 Protein (Met1-Phe765), His-tagged |
| Product Overview: |
Recombinant Human TOP1 Protein (NP_003277.1) (Met1-Phe765) was produced by Baculovirus-Insect cells expression system. This protein was expressed with a polyhistidine tag at the N-terminus. |
| Source: |
Baculovirus-Insect cells |
| Species: |
Human |
| Tag: |
His |
| Formulation: |
Lyophilized from sterile 20 mM Tris, pH 7.5, 200 mM NaCl, 20 % glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Protein length: |
Met1-Phe765 |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
The recombinant human TOP1 consists of 784 amino acids and predicts a molecular mass of 93.1 kDa. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70℃. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
