| Catalog#: |
MK0745H |
| Product Name: |
Recombinant Human TNFa |
| Product Overview: |
Recombinant human TNF alpha protein (rhTNFa) Val77-Leu233 was produced in human HEK293 cells cells. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
N/A |
| Formulation: |
Bulk protein in a 0.22 µm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization. |
| Protein length: |
Val77-Leu233 |
| Purity: |
>98% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Molecular Mass: |
rhTNFa, without any tag, has a calculated MW of 17.4 kDa expressed. The predicted N-terminus is Val77. Protein migrates as 18 kDa in reduced SDS-PAGE. The gel filtration analysis indicates that only HEK293 cells expressed TNF alpha protein is trimeric form, which has a molecular mass of 54 kDa. |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
