| Catalog#: |
MK0642H |
| Product Name: |
Recombinant Human ROR1 protein, Fc & Avi-tagged, Biotinylated |
| Product Overview: |
Biotinylated Human ROR1, Fc Tag is expressed from human 293 cells (HEK293 cells). It contains AA Gln 30 - Glu 403 (Accession # AAA60275.1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
| Source: |
HEK293 cells |
| Species: |
Human |
| Tag: |
Fc&Avi |
| Formulation: |
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose. |
| Protein length: |
AA Gln 30 - Glu 403 |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
Store under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Molecular Mass: |
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 70.4 kDa. As a result of Glycosylation, the protein migrates as 79-104 kDa under reducing (R) condition, and 158-208 kDa under non-reducing (NR) condition (SDS-PAGE). |
| Stability: |
No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). |
| Endotoxin: |
Less than 1.0 EU per μg as determined by the LAL method. |
| Notes: |
Research Use Only. Not for use in clinical procedures.Aoid repeated freeze-thaw cycles. |
