| Catalog#: |
MK0950H |
| Product Name: |
Recombinant Human PRL Protein, Fc-Avi-tagged, Biotinylated |
| Product Overview: |
Biotinylated Human Prolactin, Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Leu 29 - Cys 227 (Accession # Q5THQ0-1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag. |
| Source: |
HEK293 |
| Species: |
Human |
| Tag: |
Fc-Avi |
| Formulation: |
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5, 10% trehalose. |
| Bio-activity: |
Immobilized Human Prolactin R / PRLR Protein, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human Prolactin, Fc Tag, Avi Tag with a linear range of 5-156 ug/mL. |
| Purity: |
>95% as determined by SDS-PAGE. |
| Storage: |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Reconstitution: |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| Molecular Mass: |
The protein has a calculated MW of 51.2 kDa. The protein migrates as 57-66 kDa under reducing condition (SDS-PAGE) due to glycosylation. |
| Stability: |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Endotoxin: |
< 1.0 EU per μg of the protein as determined by the LAL method. |
